Camel cow horse goat
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ABSTRACT:
PROVIDER: PRJNA1300405 | ENA |
REPOSITORIES: ENA
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Project description:Camel milk is considered one of the most valuable food sources for nomadic people in arid and semi-arid areas and has also been consumed as a natural adjuvant for managing a variety of human diseases for centuries due to its nutritional values and extraordinary medicinal properties. A total of 180 milk samples were collected from Bikaneri and Jaisalmeri camels during various seasons in the Jodhpur and Sikar districts of Rajasthan for the proteomics study. Protein profiling of camel milk was done by LC-MS/MS. The protein pellets were prepared from 50 mL of pooled raw milk. The trypsinization and clean-up protocol was performed using standard methods. Then, Mass spectrometric analysis of peptide mixtures was performed using the EASY-nLC 1200 system coupled to a Thermo Fisher-Q Exactive equipped with a nanoelectrospray ion source. All samples were processed and analyzed with Proteome Discoverer (v2.4) against the Uniprot camel reference proteome database. A total of 704 protein groups were identified in the milk of Bikaneri and Jaisalmeri camels on the Uniprot Camelus dromedarius Database. There were 21 (3%) and 17 (2.4%) unique protein groups with 666 (94.6%) common proteins in the milk of Bikaneri and Jaisalmeri, respectively. In addition, 687 (97%) and 683 (98%) specific proteins were found in Bikaneri and Jaisalmeri camel milk, respectively. The camel casein components (CNs) were found as αs1-CN, αs2-CN, β- CN and κ-CN. Whey protein such as β-lactoglobulin was not found in the camel milk but α- lactalbumin was abundant. Breed variation was less observed because only 18 significantly expressed proteins were obtained in the milk of the Bikaneri and Jaisalmeri camel breeds.
2025-09-22 | PXD058940 | Pride
Project description:Comparison of goat and cow milk-derived extracellular vesicle miRNomes (microRNA expression profiles) determined usiing RNA-sequencing (NGS).
2023-05-10 | GSE227559 | GEO
Project description:Whey proteins are the leading proteins class in milk and play an essential role in the immune defense of neonatal mammals. The aim of this study was to analyze whey proteins in bovine, goat and camel milk by label free proteomics techniques. Finally, 840 proteins were identified, which considerably increasing the number of whey proteins identified in these species. The results of the PCA revealed significant differences in whey proteome patterns between bovine, goat and camel milk. Proteins such as PAEP, CST3, SERPING1, CTSB and GLG1 play an important role as markers in the classification of bovine, goat and camel milk. Statistical analysis showed that the relative abundances of many whey proteins such as ALB, LALBA, LTF and LPO were significantly different among different species. GO and KEGG functional analysis have shown that while the distribution of biological functions involved in whey proteins was relatively similar across species, they differed in terms of protein quantity. These data shed light on the quantitative differences and potential physiological functions of whey proteins across species, and may point the way to the production of specific functional whey proteins.
2022-10-29 | PXD037828 |
Project description:Demand for camel milk (CM) is increasing worldwide, due to its high nutritious value and health benefits. In this study, whole CM powders were produced by spray drying (SD) at six inlet temperatures (190°C - 250°C) and by freeze drying (FD). Physicochemical and functional properties of CM powder proteins were investigated. Both treatments had negative effect on casein solubility, while whey proteins remained soluble and slightly increased its solubility with the extent of MR. The CM powders obtained at higher inlet temperatures demonstrated improved antioxidant activity. Secondary structure of whey proteins did not differ among the samples, while surface hydrophobicity of whey proteins was higher in all SD than in FD samples, suggesting only limited denaturation of camel whey proteins at higher inlet temperatures of drying. Thus, the effects of SD under the conditions applied in our study did not decrease camel whey protein solubility, while drying procedure itself regardless of temperature decreased solubility of camel milk caseins. This study provides useful insights for optimization of CM powder production.
2021-02-25 | PXD023290 | Pride
Project description:Intestinal microflora of Xinjiang Bactrian Camel Raw sequence reads
| PRJNA704179 | ENA
Project description:The “ship of the desert”, the one-humped Arabian camel (Camelus dromedarius), has a remarkable capacity to survive in conditions of extreme heat without needing to drink water. One of the ways that this is achieved is through the actions of the antidiuretic hormone vasopressin (AVP) and the natriuretic hormone oxytocin (OXT), both of which are made in a specialised part of the brain called the hypothalamo-neurohypophyseal system (HNS), but exert their effects at the level of the kidney to, respectively, provoke water conservation and salt excretion. Interestingly, our electron microscopy studies have shown that the ultrastructure of the camel HNS changes according to season, suggesting that in the arid conditions of summer the dromedary’s HNS is in a state of permanent activation, in preparation for the likely prospect of water deprivation. Based on our camel genome sequence, we have carried out an RNAseq analysis of the camel HNS in summer and winter.
2019-05-17 | GSE131361 | GEO
Project description:Milk fat globule membrane (MFGM) proteins, which are recognized to play a variety of biological roles; nevertheless, their composition and interspecies complexity remain unknown. This study aimed to identify and quantify proteins extracted from MFGM-enriched fractions of bovine, goat and camel milk by a label free proteomics approach. We identified 1579 proteins, substantially increasing the number of MFGM proteins identified for these species. The results of functional analysis showed that the identified MFGM proteins had similar functional annotations, with cellular processes, intracellular anatomical structures and binding being the predominant GO annotations. And most of the proteins were involved in KEGG pathways such as protein processing of ribosomes and endoplasmic reticulum. Subsequently, multiple statistical methods were used to compare the differences in MFGM protein quantification among different species. The results of principal component analysis showed that bovine, goat and camel milk MFGM proteins were significantly different, among which CSN1S1, XDH, LTF and PI4KA played an important role in taxonomic identification. Meanwhile the results of hierarchical clustering showed that the differences (p<0.05) between bovine and goat milk were smaller than those of camel milk. These new data deepen the understanding of the protein composition of MFGM and its possible physiological roles, providing directions for the production of specific functional milk proteins.
2022-10-24 | PXD037715 |
Project description:In this project, spray drying and low temperature vacuum drying were used to produce camel milk powder, and the effects of two technologies on the abundance and biological function of bioactive proteins in camel milk were compared. The results showed that low temperature vacuum drying retained more active proteins, which was related to immune response-related functions and directly affected the nutritional value of camel milk powder.
2025-09-11 | PXD068282 |