Project description:The human microbiota is believed to influence health. Microbiome dysbiosis may be linked to neurological conditions like Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS) and Huntington’s disease (HD). We report the ability of a probiotic bacterial strain in halting neurodegeneration phenotypes. We show that Lacticaseibacillus rhamnosus HA-114 is neuroprotective in C. elegans models of ALS and HD. Our results show that neuroprotection from L. rhamnosus HA-114 is unique from other L. rhamnosus strains, and resides in its fatty acid content. Neuroprotection by L. rhamnosus HA-114 requires acdh-1/ACADSB, kat-1/ACAT1 and elo-6/ELOVL3/6, which are key fatty acid metabolism and mitochondrial b-oxidation genes. Our data suggest that disrupted lipid metabolism contributes to neurodegeneration and that dietary intervention with L. rhamnosus HA-114 restores lipid homeostasis and energy balance through mitochondrial b-oxidation. L. rhamnosus HA-114 is suitable for human consumption opening the possibility of modifying disease progression by dietary intervention.
Project description:Identification of proteins contained in extracellular vesicles of Lacticaseibacillus rhamnosus PCM 489. Dataset is related to publication http://dx.doi.org/10.20517/evcna.2024.49. This work was financially supported by the National Science Centre, Poland (no. 2021/43/D/NZ6/01464).
Project description:This project investigates the proteomic response of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus to postbiotic metabolites derived from Lacticaseibacillus rhamnosus. Bacterial cultures were exposed to sub-MIC concentrations of L. rhamnosus cell-free supernatant (CFS) or lactic acid (LA) for 6 h, and intracellular proteins were extracted for quantitative label-free proteomics using HPLC–MS/MS. The dataset includes RAW mass spectrometry data and MaxQuant output files. The aim of this study is to compare stress responses triggered by complex postbiotic mixtures versus LA alone, focusing on antimicrobial and antibiofilm potential.
