Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1304166 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Interventions: Gold Standard:Gastroscopy, colonoscopy, pathological diagnosis;Index test:Plasma exosome extraction: commercial plasma exosome extraction kit (EZB company) extraction.
Extraction of plasma exosomal miRNA: Advanced probe method RT-qPCR extraction kit (Thermo Company).
Primary outcome(s): exosome microRNA
Study Design: Cohort study
| 40619 | ecrin-mdr-crc
Project description:The microRNA (miRNA) expression profile of plasma exosome in pregnant women complicated with gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small RNA sequencing in 12 GDM and 12 normal glucose tolerance (NGT) pregnant women and validated in 102 GDM and 101 NGT pregnant women. A total of 22 exosomal miRNAs were found and five of them were verified by qRT-PCR. Exosomal miR-423-5p was upregulated, while miR-99a-5p, miR-192-5p, miR-148a-3p, and miR-122-5p were downregulated in pregnant women complicated with GDM.
2022-11-09 | GSE192813 | GEO
Project description:Purpose: To identify a panel of plasma exosomal miRNAs as potential biomarkers for lung cancer from the genome-wide set of currently known human miRNAs. Methods: Plasma samples from 4 lung cancer patients and 4 patients with benign lung disease were collected, and plasma exosomes were extracted by ultracentrifugation. Exosomal miRNA profiles were generated by deep sequencing based on Illumina NextSeq 500 platform. And differentially expressed exosomal miRNAs between lung cancer and benign lung disease were identified by edgeR with the default threshold of |log2 (fold change) | ≥ 1.5 and P-value < 0.05. Results: A total of 983 detectable exosomal miRNAs were analyzed to identify differentially expressed miRNAs between two groups. There were 6 significantly up-regulated miRNAs and 63 significantly down-regulated miRNAs in lung cancer cases when compared with benign lung disease. Conclusion: The comparative analysis of plasma exosomal miRNAs between lung cancer and benign lung diseases provided a reference for exploring potential biomarkers of lung cancer. And the clinical value of candidate exosomal miRNAs needs to be further verified in lung cancer clinical trials.
2025-02-04 | GSE196159 | GEO
Project description:The pathophysiological mechanism(s) underlying PMI remain controversial. Small non-coding molecules that modulate gene expression may enhance the detection of myocardial injury and provide further mechanistic insight. We profiled plasma exosomal microRNA in patients who sustained PMI after elective non-cardiac surgery
2020-07-26 | GSE144777 | GEO
Project description:Dengue virus infection is recognized as one of the most important mosquitos borne human diseases of the 21st century. It can manifest clinically as a moderate febrile illness, or a severe life-threatening disease called dengue haemorrhagic fever. Notably, there are currently no predictive diagnostic values for individuals prone to bleeding manifestations associated with this infection. This study explores the potential solution by focusing on exosomes, lipid vesicles enriched in specific microRNA. These exosomal microRNAs have the potential to form a disease-specific diagnostic signature, paving the way for an analysis of exosomal plasma microRNA expression to predict bleeding tendency in dengue infection. In total, 6 blood plasma exosome samples, dengue without warning sign (n = 3) and dengue with warning sign (n = 3) were used to isolate total RNA. Small RNA analysis and microarray-based expression profiling was used to detect the miRNA profile of plasma exosomes to identify predictive biomarker for bleeding manifestations in patients with warning sign as compared to patients without warning signs. Microarray profiling revealed that 24 miRNAs were upregulated in the plasma derived exosome miRNA in the dengue patients with warning signs. Functional analysis of target genes of these miRNAs in silico indicated their involvement in bleeding manifestation in dengue infection.
2025-09-12 | GSE307678 | GEO
Project description:Exosomes and exosomal miRNAs from the plasma of volunteers were isolated from thyroid nodules and papillary tyriod cancer patients . Profiling of exosomal miRNA was performed using next-generation sequencing(NGS) to identify miRNA candidates for diagnosis.
2019-05-01 | GSE130512 | GEO
Project description:Exosomes, endosome-derived membrane microvesicles, contain a specific set of RNA transcripts that are involved in cell-cell communication and hold a great potential as disease biomarkers. To systemically characterize exosomal RNA profiles, we performed RNA sequencing analysis using three human plasma samples and evaluated efficacies of small RNA library preparation protocols from 3 manufacturers. We tested the six samples (A1 and A2, B1 and B2, C1 and C2) using two small RNA library preparation kits: NEBNext Multiplex Small RNA library Prep Set from New England Biolab (NEB) and NEXTflex Small RNA Sequencing Kit from Bioo Scientific (BS). We also tested IlluminaM-bM-^@M-^Ys TrueSeq Small RNA Sample Preparation Kit (ILMN) in sample A1 and A2. Together, we tested these plasma samples by sequencing 14 indexed libraries. This study allowed direct comparison of current small RNA library preparation protocols and identified the most suitable strategy for future exosomal RNA sequencing analysis.
2013-05-15 | E-GEOD-45722 | biostudies-arrayexpress
Project description:Plasma exosomal miRNA may differ between adolescent idiopathic scoliosis patients and healthy individuals. Sequencing analysis was used to find these differential miRNAs.
2024-05-15 | GSE235203 | GEO