Project description:Using microarray technology, we aim to identify in vitro host gene expression for understanding the crosstalk of signaling pathways that leads to the joint pathogenesis in Avian orthoreovirus infection.
2018-05-09 | GSE103067 | GEO
Project description:Detection and Characterization of a Reassortant Mammalian Orthoreovirus Isolated from Bats in Xinjiang, China
Project description:We report the application of Illumina short RNA sequencing for characterization and discovery of miRNAs and moRNAs in two Italian Large White pig backfat tissue.
Project description:Viral transcriptional regulators (vTRs) have emerged as potent factors that shape the host anti-viral gene expression programs. Delineating the molecular mechanisms by which vTRs inhibit or promote transcription would provide fundamental insights for developing anti-viral strategies. Using Mammalian orthoreovirus (REOV) as a model system, we identified a new viral mechanism by which viruses antagonize the host innate immune response. We found that the REOV outer capsid protein σ3 functions as a vTR to suppress NF-κB gene expression via its direct interaction with the host helicase DHX9. Mechanistically, σ3 impairs the initial recruitment of Pol II by disrupting the interaction between DHX9 and Pol II. More interestingly, σ3 suppresses DHX9 helicase activity, resulting in the aberrant accumulation of R-loops at promoter-proximal regions, thereby affecting Pol II pause-release and ultimately suppressing NF-κB gene expression. Together, our findings reveal an unprecedented strategy employed by a viral protein that regulates anti-viral gene expression by directly modulating the host transcription factor DHX9.
Project description:Expression profiles of porcine muscle Semimembranosus were studied to identify genes beeing significantly affected by pre-slaughter stress or no stress treatment in pigs of different breed (Italian Duroc, Italian Large White or Pietrain) and differnt Halothane genotype (RYR1 locus CC-NN, CT-Nn or TT-nn) in Pietrain pigs.
