Project description:Utilizing the 10X Genomics Cell Ranger (Pipeline Version 3.1.0) platform, this study investigates the impact of osteocyte ablation on skin aging. Skin and bone share significant structural and molecular homology, including compositional architecture, cellular origins, and signaling pathways. Both tissues exhibit parallel degenerative changes during natural aging and under pathological conditions, characterized by collagen depletion leading to osteoporosis and skin atrophy. Through scRNA-seq analysis of skin following osteocyte knockout, our research provides novel insights into the regulatory role of osteocytes in skin aging.
Project description:We studied the membrane protein compositions of Streptococcus pneumoniae WT and scRNA mutant strains, with or without the CSP1 induction into competence state.
Project description:In this study, we analysed early embryonic skin development (mus musculus; C57BL/6J) at the transcriptional level. Major questions concerned the cell type composition of early embryonic skin, and the emergence of transcriptional heterogeneity among epithelial and stromal precursor cells. Cells were isolated from embryonic dorsal skin and randomly sequenced (scRNA-Seq using 10X Genomics v2) without any cell sorting. Data from three embryonic time points (E12.5, E13.5, and E14.5) was integrated and compared to obtain a better understanding of the dynamics of early skin development.
Project description:To investigate the effect of iNKT deficiency in the skin, the analysis of global gene expression in the skin tissues from WT and Cd1d KO mice were performed using RNA-seq data obtained from these samples.