Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CUT&Tag-seq of H3k27me3 in vehicle and DOPAC-treated CD8+ T cells

ABSTRACT: CUT&Tag-seq of H3k27me3 in vehicle and DOPAC-treated CD8+ T cells

PROVIDER: PRJNA1304407 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

CUT&Tag-seq of H3k27me3 in vehicle and DOPAC-treated CD8+ T cells

Project description:To investigate epigenetic alterations in vehicle and DOPAC-treated CD8+ T cells

2025-08-16 | GSE305082 | GEO

H3K27me3 localization in UTX deficient Plasma cells by CUT&Tag

Project description:To understand the role of the H3K27me3 demethylases, UTX and JMJD3, in B cell differentiation. CUT&Tag for H3K27me3 was performed on CreCtrl and dKO (UTX and JMJD3-deficient) PC at day three post in vivo stimulation with LPS.

2022-02-01 | GSE185098 | GEO

CUT&Tag analysis of H3K4me3, H3K27Ac,H2A119Ub and H3K27me3 in treated KPC1199 murine PDAC cell line

Project description:To gain mechanistic insight into how Epigenetic factors reprogramming tumor immune microenvironment in response to IFNγ stimulation. we performed CUT&Tag sequencing in murine PDAC cells.For comparison of the transcription profile between IFNγ-PD-L1all and IFNγ+PD-L1all cells, we treated KPC1199 cells with vehicle control or 10 ng/mL recombinant mouse IFNγ for 20 hours and harvested them. For comparison of PD-L1hi with PD-L1all, cells were either sorted with the top 15% PD-L1+ or total of IFNγ+PD-L1all PDAC cells, and freshly collected as soon as possible. Hyperactive pA-Tn5 Transposase for CUT&Tag kit from Vazyme (TD901), and antibodies against H3K4me3 from Abcam (ab213224), H3K27Ac from Abcam (ab4729),H2A119Ub from Cell Signaling (8240T) and H3K27me3 from Abcam (ab6002) were employed. Trueprep index kit v2 and v3 for illumina were used to establish DNA library.

2024-08-20 | GSE222133 | GEO

CUT&Tag analysis of H3K36me3 and H3K27me3, in Setd2-WT and -KO murine PDAC cell line

Project description:To gain mechanistic insight into how Setd2 loss promotes activation of AKT signaling, we performed CUT&Tag sequencing in PDAC cells (KPC1199) with Setd2-KO and Setd2-WT). Setd2-WT and -KO murine PDAC cells were cultured and freshly collected as soon as possible. Hyperactive pA-Tn5 Transposase for CUT&Tag kit from Vazyme (TD901), and antibodies against H3K36me3 and H3K27me3 were employed. Trueprep index kit v2 and v3 for illumina were used to establish DNA library.

2023-02-06 | GSE176013 | GEO

YAP-Driven Malignant Reprogramming of Oral Epithelial Stem Cells, CUT&TAG sequencing for YAP, H3K27ac, and H3K27me3

Project description:Cleavave Under Targets and Tagmentation (CUT&TAG) sequencing for YAP, H3K27ac, and H3K27me3 of tongue epithelia-derived, transgene-selected primarily cultured cells from control mice or transgenic mice expressing HPV16E6-E7 and YAP1S127A

2024-09-09 | GSE276779 | GEO

CUT&Tag analysis of H3K4me3 and H3K27me3 in the neural stem/progenitor cells under crotonate treatment.

Project description:To gain a deeper insight into how crotonate regulates embryonic neural stem/progenitor cells (NSPCs) proliferation and differentiation, CUT&Tag was performed to analyze the genome-wide changes of H3K4me3 and H3K27me3 stimulated with crotonate in E13.5 NSPCs.

2021-06-15 | GSE178097 | GEO

H3K27me3 conditions chemotolerance in triple-negative breast cancer [CUT&TAG]

Project description:H3K27me3 conditions chemotolerance in triple-negative breast cancer [CUT&TAG]

| PRJNA776906 | ENA

H3K27me3 localization in UTX deficient Plasma cells by CUT&Tag

Project description:H3K27me3 localization in UTX deficient Plasma cells by CUT&Tag

| PRJNA767652 | ENA

Cut&Tag of mESCs in 2i, 30hr N2B27/DMSO/siNegative, 30hr N2B27MEK(i)/siNanog cells

Project description:Cut&Tag using antibodies against H3K27me3, H3K4me1, H3K4me3, HeK27ac, IgG Cells: mouses embryonic stem cells (mESCs), RGd2 Culture conditions: 2i, 30hr N2B27/DMSO/siNegative, 30hr N2B27MEK(i)/siNanog

2024-07-17 | E-MTAB-14031 | biostudies-arrayexpress

PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation [CUT&Tag, CUT&RUN]

Project description:To understand the functional role of PRC1 in defining the epigenomic and transcriptional landscape in Aundiff, we eliminated PRC1 function in germ cells using Ddx4-Cre to mutate the E3 ubiquitin ligase RNF2 on a background lacking its partially redundant paralog RING1 (RING1A) to create PRC1 conditional knockout (PRC1cKO) mice. We discover a key role of Polycomb complexes in shielding the undifferentiated state of mouse adult spermatogonia. CUT&RUN or CUT&Tag for H2AK119ub, H3K27me3 and H3K4me3 in WT, PRC1ctrl and PRC1cKO spermatogonial cells.

2023-12-18 | GSE221942 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data