Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1305107 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:<p>This study explores the impact of psychological factors on the comorbidity of dental caries and obesity in adolescents through the oral-gut-brain axis. An epidemiological survey was conducted on 1,024 students from Beizhen No. 1 Junior High School, with 90 adolescents aged 12–15 included in biosample research. Methods such as 16S rRNA gene sequencing, untargeted metabolomics, and SourceTracker analysis were used. Results showed significant differences in oral and gut microbiota and metabolite concentrations among adolescents with different health statuses, highlighting the roles of the oral-gut and oral-brain axes in disease progression. This study provides new insights into the mechanisms underlying the comorbidity of dental caries and obesity in adolescents and offers theoretical foundations for prevention and treatment strategies.</p>
2025-08-22 | MTBLS12873 | MetaboLights
Project description:We conducted a cross-sectional study involving 85 adolescents aged 12–15 years, divided into healthy controls (HC), caries-obesity comorbidity (CO), and caries-obesity with sleep deprivation (COS) groups. Oral and fecal samples were collected for 16S rRNA gene sequencing, untargeted metabolomics, and SourceTracker analysis. Additionally, an animal study using Sprague-Dawley rats was performed to validate the findings, incorporating behavioral tests, Micro-CT, serum biochemistry, histology, and intestinal transcriptomics.
2026-04-29 | MTBLS14395 | MetaboLights
Project description:The aim of this study was to compare the protein composition of the acquired pellicle from adolescents with Erosive Tooth Wear (ETW) caries and sound. Calibrated examiners in BEWE Index and ICDAS-merged Epi criteria assessed ETW and caries in a sample of 454 systemically healthy adolescents aged 12 to 15 years old. From them, 30 subjects were selected for this study, presenting either Sound enamel (n=10, Total BEWE sum <8 and without extensive/cavitated caries lesions), ETW (n=10, Total BEWE sum ≥9 and without extensive/cavitated caries lesions) or Caries (n=10, Total BEWE sum <8 and with at least one Extensive caries lesion). Two hours formation acquired pellicle samples were taken from tooth surfaces (Buccal/Occlusal/Palatal/Lingual). Protein composition was analysed by Liquid Chromatography Tandem Mass Spectrometry. Using mean reporter ion values relative abundances of proteins were compared among the three groups to calculate for fold changes. Protein increases or decreases of 2-fold were reported (t-test, p<0.05). Gene Ontology (GO) of the included proteins was assigned. In the results of this project were found a total of 127 proteins in the acquired pellicle. The GO analyses showed that the majority of detected proteins were stress-response related. The ETW group disclosed up-regulated relative abundance of Antileucoprotease; Histatin and Prolactin-induced protein Hemoglobin subunits Alpha (HBA) and Beta (HBB) showed decreased relative abundances in the ETW and Caries groups when compared to the Sound group. As a conclusion, the acquired pellicle from individuals with ETW showed differences when compared to other dental conditions, with an increase in relative abundance of some stress response associated proteins in ETW and a decrease in proteins related to salivary protection against acid challenges.
2026-01-16 | PXD053912 | Pride
Project description:The study aims to assess gene expression in plaque samples collected from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis. File Naming Conventions are as follows: Patient ID : 4 digit identifier Diagnosis : Caries Negative(CN) or Caries Positive(CP) Type of Twin: Monozygotic(MZ)or Dizygotic(DZ) Pair to xxxx: 4 digit twin identifier maps to the Patient ID E.g: 2126_CP_MZ_PairTo_2125_fastqc - 2126 is a caries positive patient and pairs to monozygotic twin pair 2125. Plaque samples from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis are enriched for bacterial messenger RNA to study the gene expression differences in the samples. RNA was extracted from RNAprotect (Qiagen, In c.) treated dental plaque scrapings from 38 patients. Amplified cDNA was created and rRNA sequence was removed by subtractive hybridization. Individual patient samples were run on a single lane of an Illumina Genome Analyzer.
2011-09-26 | E-GEOD-31439 | biostudies-arrayexpress
Project description:The study aims to assess gene expression in plaque samples collected from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis. File Naming Conventions are as follows: Patient ID : 4 digit identifier Diagnosis : Caries Negative(CN) or Caries Positive(CP) Type of Twin: Monozygotic(MZ)or Dizygotic(DZ) Pair to xxxx: 4 digit twin identifier maps to the Patient ID E.g: 2126_CP_MZ_PairTo_2125_fastqc - 2126 is a caries positive patient and pairs to monozygotic twin pair 2125. Plaque samples from twin pairs that are both concordant and discordant with respect to dental Caries diagnosis are enriched for bacterial messenger RNA to study the gene expression differences in the samples.
2011-09-26 | GSE31439 | GEO
Project description:Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In this study, we observed supplementation with 1.5% arginine (final concentration) had inhibitory effects on the growth of S. mutans in complex and chemically defined media, particularly when cells were exposed to acid or oxidative stress. Deep-sequencing of RNA (RNA-Seq) comparing the transcriptomes of S. mutans growing in a chemically defined medium with and without 1.5% arginine in neutral and acidic pH conditions and under oxidative stress conditions revealed interesting results. The results provide new insights into the mechanisms of action by which arginine inhibits dental caries through direct adverse effects on multiple virulence-related properties of the most common human dental caries pathogen. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen.
2017-05-20 | GSE98020 | GEO
Project description:We present a collection of single-cell transcriptomic profiles of 6,810 pulpal cells isolated from a sound human maxillary third molars and carious teeth at different stages. We showed that the presence of deep, but not enamel caries, altered the immune cell compositions of the dental pulp. Differential expression analysis further revealed that the pro-inflammatory, anti-inflammatory and mineralization-related genes were upregulated in immune and stromal cells in deep dental caries. Cell-cell interaction prediction showed potential interactions between immune and stromal cells during homeostasis, and enhanced interactions between different cell types with macrophage during deep dental caries. Taken together, our study serves as a comprehensive survey of human pulpal cell heterogeneity, as well as provides novel molecular insights into dental pulps in health and disease.
2021-11-26 | GSE185222 | GEO
Project description:Objectives: Dental pulp stem cells are crucial in immune response regulation. However, sub-clusters of these cells involved in deep caries progression remain unidentified. This study explores the role of Intercellular adhesion molecule 1-positive dental pulp stem cells (ICAM1+DPSCs) within the immune microenvironment of dental pulp tissue affected by deep caries, aiming to establish a theoretical foundation and novel strategies for its prevention and treatment.Methods: Use flow cytometry to sort ICAM1+ DPSCs and ICAM1- DPSCs for RNA-seq.
2026-01-01 | GSE282493 | GEO