UV induced translation capacity is developmentaly regulated in fungi
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ABSTRACT:
PROVIDER: PRJNA1305407 | ENA |
REPOSITORIES: ENA
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Project description:In fast-growing cells, most RNA molecules encode ribosomal subunits. During growth arrest caused by DNA damage, ribosome biogenesis is reduced because it is an highly energy demanding process. Here we report that, in two filamentous fungi, UV radiation significantly induces ribosome levels and translation capacity. This UV-induced response is developmentally regulated, occurring mosly after filament formation. Translation induction modestly enhances both UV survival and repair. Most of the mRNAs that are differentialy associated with ribosomes following UV exposure belong to modules involved in protein folding and gene expression, not only DNA repair. Furthermore, UV-induced translation is dependent on PKA signaling but remains unaffected by Target Of Rapamycin inhibition. These findings suggest a novel type of fungal response to DNA damage, where the primary aim is to protect the integrity of tgene expression from the detrimental effects of UVon transcription
2025-08-16 | GSE305365 | GEO
Project description:We previously revealed developmental regulation over UV repair capacity in the soilborne pathogen Fusarium oxysporum (F. oxysporum). We demonstrated that photoreactivation assisted survival and repair were high during early stages of germination and low at later stages. In agreement, the expression of the UV specific repair genes photolyase and uvde showed opposite trends. While early on photolyase is induced and uvde is reduced, the trend is reversed later. Here, we tested the dynamic of transcription of photolyase, UV survival, repair capacity, and UV induced mutagenesis in the foliar pathogen Fusarium mangiferae. Unlike F. oxysporum, neither did we observe developmental control over photoreactivation dependent repair nor the changes in gene expression of phr1 and uvde throughout the experiment. Similarly, photo-reactivation assisted reduction in UV induced mutagenesis was similar throughout the development of F. mangiferae but fluctuated during the development of F. oxysporum. To generate hypotheses regarding the recovery of F. mangiferae after UV exposure, an RNAseq analysis was performed after irradiation at different timepoints. The most striking effect of UV on F. mangiferae was developmental-dependent induction of translation related genes. We further report a complex dynamic response that involves translation, cell cycle and lipid biology related genes.
2024-01-30 | GSE254119 | GEO
Project description:Translation is a basic cellular process and its capacity is adapted to cell function. In particular, secretory cells achieve high protein synthesis levels without triggering the protein stress response. It is unknown how and when translation capacity is increased during differentiation. Here, we show that the transcription factor Creb3l2 is a scaling factor for translation capacity in pituitary secretory cells and that it directly binds ~75% of regulatory and effector genes for translation. In parallel with this cell-autonomous mechanism, implementation of the physiological UPR pathway prevents triggering the protein stress response. The pituitary differentiation factor Tpit activates Creb3l2 expression, the Creb3l2-dependent regulatory network as well as the physiological UPR pathway. Thus, Creb3l2 implements high basal translation levels through direct targeting of translation effector genes acting downstream of signaling pathways that otherwise regulate protein synthesis. Expression of Creb3l2 may be a useful means to enhance production of therapeutic proteins.
2019-07-29 | GSE132320 | GEO
Project description:Translation is a basic cellular process and its capacity is adapted to cell function. In particular, secretory cells achieve high protein synthesis levels without triggering the protein stress response. It is unknown how and when translation capacity is increased during differentiation. Here, we show that the transcription factor Creb3l2 is a scaling factor for translation capacity in pituitary secretory cells and that it directly binds ~75% of regulatory and effector genes for translation. In parallel with this cell-autonomous mechanism, implementation of the physiological UPR pathway prevents triggering the protein stress response. The pituitary differentiation factor Tpit activates Creb3l2 expression, the Creb3l2-dependent regulatory network as well as the physiological UPR pathway. Thus, Creb3l2 implements high basal translation levels through direct targeting of translation effector genes acting downstream of signaling pathways that otherwise regulate protein synthesis. Expression of Creb3l2 may be a useful means to enhance production of therapeutic proteins.
2019-07-29 | GSE132322 | GEO
Project description:Hyperaccurate mitochondrial translation increases cellular proteostatic capacity
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Project description:Translation is a basic cellular process and its capacity is adapted to cell function. In particular, secretory cells achieve high protein synthesis levels without triggering the protein stress response. It is unknown how and when translation capacity is increased during differentiation. Here, we show that the transcription factor Creb3l2 is a scaling factor for translation capacity in pituitary secretory cells and that it directly binds ~75% of regulatory and effector genes for translation. In parallel with this cell-autonomous mechanism, implementation of the physiological UPR pathway prevents triggering the protein stress response. The pituitary differentiation factor Tpit activates Creb3l2 expression, the Creb3l2-dependent regulatory network as well as the physiological UPR pathway. Thus, Creb3l2 implements high basal translation levels through direct targeting of translation effector genes acting downstream of signaling pathways that otherwise regulate protein synthesis. Expression of Creb3l2 may be a useful means to enhance production of therapeutic proteins.
2019-07-29 | GSE132321 | GEO
Project description:Transcriptome analysis of ultraviolet-radiation time course with paired treatment and control measurements provided groups of coordinately regulated genes and Gene Ontology processes. A set of genes significantly affected by UV was selected by linear modeling plus order-restricted inference profile matches; this gene list was used to find upstream sequence motifs that predict UV regulation of gene expression. Keywords: time course dose response stress response
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Project description:Epigenetic programming by trithorax supports the translation capacity of Drosophila memory neurons.
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Project description:Cell-autonomous transcriptional mechanism for enhancement of translation capacity in secretory cells
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Project description:Although messenger RNA (mRNA) vaccines have been employed to prevent the spread of COVID-19, they are critically limited by instability and low translation capacity. Alterations in tRNA abundance and modification, associated with codon optimality, impact mRNA stability and protein output in a codon-dependent manner, implying tRNA as a potential translation enhancer. Here, we report a strategy named tRNA-plus to augment translation based on artificially modulating the tRNA availability. We demonstrated that overexpression of specific tRNAs enhanced the stability and translation efficiency of SARS-CoV-2 Spike mRNA, resulting in substantial improvements in protein levels by up to 4.7-fold. In addition, chemically synthesized tRNAs bearing multiple site-specific modifications, particularly at the anticodon loop and TΨC-loop, exhibited an average of ~4-fold higher decoding efficacy than unmodified tRNA, along with increased stability and reduced immune toxicity. We further leveraged an optimized lipid nanoparticles (LNP) system for codelivery of Spike mRNA vaccine and tRNA, which elicited enhanced spike-specific humoral and cellular immune responses in vivo. The methods presented here may become a general approach for elevating mRNA translation potency, which can be applied to various translation-based fields.
2025-07-08 | PXD065927 |