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CaSR activation triggers mandibular overgrowth in fMP patients and mice

ABSTRACT: CaSR activation triggers mandibular overgrowth in fMP patients and mice

PROVIDER: PRJNA1306058 | ENA |

REPOSITORIES: ENA

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Project description:Mandibular prognathism (MP) is the most common type of dento-maxillofacial deformity in East Asian populations. Genetic studies have revealed several MP-associated loci, suggesting that MP could be inherited as familial MP (fMP). However, functional verifications and in-depth mechanistic investigations of these loci are limited. For this study, we recruited 5 fMP families with 17 fMP members and 7 normal members. We first compared the clinical features of the 17 fMP members with 31 non-familial MP patients, finding a stronger mandibular overgrowth phenotype in the fMP subjects. Next, we performed whole-exome sequencing (WES) analysis with members of the 5 fMP families and singled out a potential fMP-associated pathogenic variant in the CASR gene—namely, rs117375173—the mutation introduces an amino acid substitution (A601G) in exon 7 and confers gain-of-function in Calcium-Sensing Receptor (CaSR). The rs11735173 variant changes the CaSR protein structure toward a semi-active state, similar to CaSR activated by L-tryptophan (L-Trp). To verify the regulating roles of CASR in mandibular bone growth, we further generated different mouse models with abnormal CaSR function. L-Trp administration effectively activate CaSR/GNAQ expression in vivo and in vitro. The MC3T3-E1 cell line transfected with CaSR with rs117375173 showed increased osteogenic differentiation and collagen synthesis at transcriptional level. Local injection of L-Trp in the mandible of growing mice significantly increased the mandibular length and BMD, due to activated osteogenic activity and suppressed bone resorption. At the same time, loss-of-function of CaSR in osteogenic progenitors caused mandibular growth retardation in Gli1-CreER; Casrfl/fl; tdTomatofl/+ mice. In conclusion, our study reveals that abnormal functioning of CaSR affects mandibular bone development and may contribute to the pathogenesis of fMP, providing a theoretical and experimental basis for the early diagnosis of and therapeutic strategies for fMP in clinical practice.

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CaSR activation triggers mandibular overgrowth in fMP patients and mice

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