Project description:Background and Aims: Lifespan is influenced by complex interactions between genetic and environmental factors. Limitations in studying those factors in model organisms of a single genetic background hinder translational value. Here, we mapped genetic determinants of lifespans in 85 C. elegans recombinant intercross advanced inbred lines (RIAILs). We assessed molecular profiles – transcriptome, proteome, and lipidome – and life-history traits (lifespan), development, growth dynamics, and reproduction.
Project description:Recombinant inbred lines were created by crossing the alpha-synuclein containing Caenorhabditis elegans strains NL5901 and SCH4856. These strains contain the human alpha-synuclein gene fused to YFP and under the control of an unc-54 promotor (unc-54p::alpha-synnuclein::YFP) in an N2 and CB4856 genetic background, respectively. These two strains were used to generate a total of 212 recombinant inbred lines, of which 88 were genotyped by whole-genome sequencing using a MiSeq. These recombinant inbred lines can be used for mapping genetic modifiers affecting protein accumulation.
Project description:We mapped quantitative trait loci for genome-wide gene expression (eQTL) in juvenile, reproductive and senescent C. elegans recombinant inbred lines, to determine heritable transcript dynamics
Project description:The glp-1/NOTCH pathway is a conserved pathway that plays an important role in developmental control. To study the effect of natural genetic variation on perturbations in this pathway, we used N2xCB4856 recombinant inbred lines of the nematode Caenorhabditis elegans. These were treated with empty vector or gld-1 RNAi, where gld-1 is a key developmental gene in the glp-1/NOTCH pathway. The recombinant inbred lines were exposed for two generation to the treatment and 47 hour old L4 juveniles were collected for RNA isolation. In total 39 RILs were exposed to the empty-vector treatment and 46 RILs were exposed to the gld-1 treatment. Gene expression was quantified using microarrays.
Project description:We mapped quantitative trait loci for genome-wide gene expression (eQTL) in juvenile, reproductive and senescent C. elegans recombinant inbred lines, to determine heritable transcript dynamics in total 54 dual color microarrays were done on three age groups.
Project description:We analyzed the consequences of recombination in gene expression. For this, we measured expression patterns in two C. elegans wild types, N2 and CB4856 (CB). We compare these profiles with gene expression values from a Recombinant Inbred Population (RIL) derived from the same wild types. Expression values of the RILs (GSE17071) were obtained from Viñuela et al. (Genome Research, 2010). N2 and CB strain nematodes were cultured in identical conditions. Likewise, mRNA was extracted at three different ages (juveniles, reproductive and old worms) to investigate the transcriptional consequences of recombination in aging worms. Then, we explored gene expression heritability and transgression as genetic parameters for the analysis of gene expression divergence in natural isolates. Moreover, we investigated the progression of those parameters with age.
