Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
2025-06-15 | GSE298403 | GEO
Project description:western subarctic pacific ocean metagenomic data
Project description:White muscle samples from juvenile Pacific halibut were analyzed in discovery-driven proteomics to investigate the effects of temperature-induced growth manipulations and to identify protein markers of somatic growth. Juvenile Pacific halibut were either acclimated to 2C (low temperature) and 9C (control temperature) to elicit growth suppression and also initially acclimated to 2C and subsequently transferred to 9C to elicit growth compensation. This project was led by Josep Planas at the International Pacific Halibut Commission.
Project description:The razor clam Sinonovacula constricta (Lamarck 1818) is a famous marine bivalve species that widely distributed along the western Pacific coast with important economical and nutritional values. During the evolution, S. constricta has formed specific biological features to adapt to its living habit. To clarify the underlying molecular mechanism in forming the specific biological features, the tissues of siphon, gill, labial palp, foot, mantle, and intestine of S. constricta were subjected to RNA-sequencing. The results showed significant differences existed in gene expression among different tissues, which provided a molecular framework for understanding the differentiation of S. constricta tissues and their underlying specific biological functions.
Project description:The diazotroph Trichodesmium is an important contributor to marine dinitrogen (N2) fixation, supplying so-called new N to phytoplankton in typically N-limited ocean regions. Identifying how iron (Fe) and phosphorus (P) influence Trichodesmium activity and biogeography is an ongoing area of study, where predicting patterns of resource stress is complicated in part by the uncertain bioavailability of organically complexed Fe and P. Here, a comparison of 26 metaproteomes from picked Trichodesmium colonies identified significantly different patterns between three ocean regions: the western tropical South Pacific, the western North Atlantic, and the North Pacific Subtropical Gyre. Trichodesmium metaproteomes across these regions significantly differed in KEGG submodule signals, and vector fitting showed that dissolved Fe, phosphate, and temperature significantly correlated with regional proteome patterns. Populations in the western tropical South Pacific appeared to modulate their proteomes in response to both Fe and P stress, including a comparatively low relative abundance of the N2 fixation marker protein, NifH. Significant increases in the relative abundance of both Fe and P stress marker proteins previously validated in culture studies suggested that Trichodesmium populations in the western North Atlantic and North Pacific were P-stressed and Fe-stressed, respectively. These patterns recapitulate established regional serial and co-limitation patterns of resource stress on phytoplankton communities. Evaluating community stress patterns may therefore predict resource controls on diazotroph biogeography. These data highlight how Trichodesmium modulates its metabolism in the field and provide an opportunity to more accurately constrain controls on Trichodesmium biogeography and N2 fixation.