Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1308820 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Understanding the environmental factors that shape microbial communities is crucial, especially in extreme environments, like Antarctica. Two main forces were reported to influence Antarctic soil microbes: birds and plants. Both birds and plants are currently undergoing unprecedented changes in their distribution and abundance due to global warming. However, we need to clearly understand the relationship between plants, birds and soil microorganisms. We therefore collected rhizosphere and bulk soils from six different sampling sites subjected to different levels of bird influence and colonized by Colobanthus quitensis and Deschampsia antarctica in the Admiralty Bay, King George Island, Maritime Antarctic. Microarray and qPCR assays targeting 16S rRNA genes of specific taxa were used to assess microbial community structure, composition and abundance and analyzed with a range of soil physico-chemical parameters. The results indicated significant rhizosphere effects in four out of the six sites, including areas with different levels of bird influence. Acidobacteria were significantly more abundant in soils with little bird influence (low nitrogen) and in bulk soil. In contrast, Actinobacteria were significantly more abundant in the rhizosphere of both plant species. At two of the sampling sites under strong bird influence (penguin colonies), Firmicutes were significantly more abundant in D. antarctica rhizosphere but not in C. quitensis rhizosphere. The Firmicutes were also positively and significantly correlated to the nitrogen concentrations in the soil. We conclude that the microbial communities in Antarctic soils are driven both by bird and plants, and that the effect is taxa-specific.
2012-06-01 | GSE33847 | GEO
Project description:Pseudomonas fluorescens SBW25 cultures were inoculated into the rhizospheres of barley seedlings of the Chevallier and Tipple varieties growing in axenic cultures. Bacterial cells were collected from the rhizosphere one and five days after inculation and RNA extracted from them. Culture used for inoculation (but not exposed to the rhizospheres) were used as control. The aim of the experiment was to determine the changes in gene expression of P. fluorescens SBW25 upon exposure to barley rhizosphere and also to determine if the rhizospehres of the two varieties of Barley had different effects on gene expression of P. fluorescens SBW25.
2023-05-15 | E-MTAB-12918 | biostudies-arrayexpress
Project description:Rhizosphere microorganisms of different alfalfa varieties in saline-alkali soil
| PRJNA1028542 | ENA
Project description:<p>Plant diseases have an important impact on the agricultural economy, often causing a decrease in crop yield and quality (Huang et al., 2020). Cruciferous plants can be used as both crops and vegetables, and have important application value. For example edible and oil sources, animal feeds and human biomedicine (Wittkop et al., 2009) (Li et al., 2022). Cruciferous crops are also susceptible to plant diseases, with clubroot being the major disease (Khalid et al., 2022). The pathogen that causes clubroot is Plasmodiophora brassicae, an obligate biotrophic protist and plasmodiophorid (Khalid et al., 2022). After plant infection P. brassicae, the root galls, stunted growth and accelerated flowering (Schuller and Ludwig-Müller, 2016). Meanwhile, P. brassicae can infect almost all cruciferous crops and lead to yields and quality losses of oil crops and vegetables worldwide (Khalid et al., 2022).</p><p><br></p><p>Here, we hypothesized that Pakchoi disease-resistant plants recruited beneficial microorganisms to reduce clubroot disease infestation. The enhanced recruitment of plant-beneficial microorganisms is hypothesized to occur via changes in Pakchoi root exudates, and some beneficial key microorganisms play a decisive role in the resistance to clubroot disease infection. To test this hypothesis, (1) we evaluated the establishment of soil microbiome in roots and rhizosphere in susceptible and disease-resistant plants; (2) to analyze the changes in the metabolome of rhizosphere soil of susceptible and resistant varieties of Pakchoi, so as to provide clues for the recruitment of beneficial bacteria; (3) the association analysis of metabolome and microbiome was used to determine the control effect of key species on clubroot disease.</p>
2025-03-31 | MTBLS10976 | MetaboLights
Project description:Arsenic (As) bioavailability in the rice rhizosphere is influenced by many microbial interactions, particularly by metal-transforming functional groups at the root-soil interface. This study was conducted to examine As-transforming microbes and As-speciation in the rice rhizosphere compartments, in response to two different water management practices (continuous and intermittently flooded), established on fields with high to low soil-As concentration. Microbial functional gene composition in the rhizosphere and root-plaque compartments were characterized using the GeoChip 4.0 microarray. Arsenic speciation and concentrations were analyzed in the rhizosphere soil, root-plaque, porewater and grain samples. Results indicated that intermittent flooding significantly altered As-speciation in the rhizosphere, and reduced methyl-As and AsIII concentrations in the pore water, root-plaque and rice grain. Ordination and taxonomic analysis of detected gene-probes indicated that root-plaque and rhizosphere assembled significantly different metal-transforming functional groups. Taxonomic non-redundancy was evident, suggesting that As-reduction, -oxidation and -methylation processes were performed by different microbial groups. As-transformation was coupled to different biogeochemical cycling processes establishing functional non-redundancy of rice-rhizosphere microbiome in response to both rhizosphere compartmentalization and experimental treatments. This study confirmed diverse As-biotransformation at root-soil interface and provided novel insights on their responses to water management, which can be applied for mitigating As-bioavailability and accumulation in rice grains.
2021-07-12 | GSE179671 | GEO
Project description:The wild olive tree (Olea europaea L. subsp. europaea var. sylvestris) plays a crucial role in the future of olive cultivation, facing challenges such as climate change and emerging diseases. The selection of resistant varieties is among the most sustainable methods for controlling Verticillium wilt. We compared the transcriptomes from two oleaster cultivars with markedly different responses to V. dahliae, AC15 and AC18, susceptible and tolerant, respectively. The differences in the gene expression profile found between AC15 and AC18 stems may determine susceptibility to the disease in the stem tissue. These differences are reflected in the lignin composition of the cell wall.
2025-03-13 | GSE245934 | GEO