Project description:This study describes a genome-wide CRISPR-Cas9 knockout (GeCKO) screen performed in two porcine cell lines, PK15 and IPEC-J2. A custom-designed porcine GeCKO (pGeCKO) library targeting protein-coding genes, lncRNAs, and miRNAs was used. Genomic DNA was harvested from GeCKO cell populations at four timepoints between 3-26 days post-transduction in each cell line. Amplicon sequencing libraries were generated from guide RNA regions using custom primers with Illumina adapter sequences, and sequenced on the NovaSeq 6000 platform (PE150). This dataset enables quantification of guide RNA abundance over time and identification of essential genes in pig cells, supporting research in comparative genomics, functional genomics, and translational animal models.
Project description:Genome-wide CRISPR screens were performed in Huh-7 hepatoma cell line to identify genes regulating ferroptosis sensitivity. Huh-7 cells were transduced with Human GeCKO library and selected by RSL-3.
