Project description:Genome-wide CRISPR screens were performed in Huh-7 hepatoma cell line to identify genes regulating ferroptosis sensitivity. Huh-7 cells were transduced with Human GeCKO library and selected by RSL-3.
Project description:To explore histamine target genes,we used CRISPR-PoolTMKOUT-Human-Single vector to build GeCKO(genome-scale CRISPR-Cas9 knockout) human library.Then Genome-wide CRISPR screen was used to detect target genes in cholangiocarcinoma cells.