Project description:The Human CRISPR Knockout Pooled Library (GeCKO v2, 1000000048, Addgene) was transferred into WISH cells. Then,half of these cells were infected with HSV-1 for 30 hours, and the remaining lived infected cells and uninfected cells were collected for sgRNA sequencing and enrichment analysis.
Project description:RNA-sequencing of PEO1 cells grown in suspension (SUS1 and SUS3) was performed to evaluate transcriptional reprogramming during anokis escape. CRISPR/Cas9 screen of PEO1 cells grown in adherent and suspension culture settings for 5 days or 10 days. GeCKO v2 library transduced in PEO1 cell line and cells were selected with puromycin. PEO1 line was authenticated prior to sequencing. PEO1 parental were confirmed to be BRCA2-mutated (5139C>G).
Project description:Reptiles exhibit a broad variety of adaptive colours and colours patterns but little is known of the mechanisms responsible for these traits. We focus here on the Hypomelanistic traits of 1 corn snake color morph and 3 leopard gecko color morphs. We use mapping-by-sequencing and and differential expression analysis to identify the causative mutation and identify the genes involved in the phenotypes of these color morphs.
Project description:Reptiles exhibit a broad variety of adaptive colours and colours patterns but little is known of the mechanisms responsible for these traits. We focus here on the Hypomelanistic traits of 1 corn snake color morph and 3 leopard gecko color morphs. We use mapping-by-sequencing and and differential expression analysis to identify the causative mutation and identify the genes involved in the phenotypes of these color morphs.