Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bulk RNA sequencing for various tissues using SENCR-BAC transgenic mice

ABSTRACT: Bulk RNA sequencing for various tissues using SENCR-BAC transgenic mice

PROVIDER: PRJNA1311519 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Bulk RNA sequencing for various tissues using SENCR-BAC transgenic mice

Project description:SENCR is a vascular-restricted long noncoding RNA specifically expressed in humans. To investigate its biological role in vivo, we generated a transgenic mouse line by introducing a ~220 kb genomic locus encompassing SENCR and its antisense neighbor FLI1, using a bacterial artificial chromosome (BAC clone: RP11-744N12) in combination with PiggyBAC technology. To assess whether SENCR-BAC mice recapitulate the human tissue-specific expression pattern, we collected multiple tissues, including the aorta, heart, brain, kidney, liver, lung, spleen, and stomach, and performed bulk RNA sequencing to evaluate SENCR expression levels across different tissues. The clean reads were mapped to genome using human genome database, but not mouse genome database, due to the objective of this experiment is focused on comparing the level of SENCR, which is human specific.

2025-09-05 | GSE306629 | GEO

Expression data from BAC transgenic mice overexpressing Glo1

Project description:We generated mice with a transgenic BAC on a B6 background. The BAC contains Glo1, and the transgenic mice were found to overexpress Glo1. We performed a microarray on whole-brain RNA of adult mice to identify differentially expressed genes resulting from Glo1 overexpression.

2012-03-27 | GSE36819 | GEO

Expression data from BAC transgenic mice overexpressing Glo1

2012-03-26 | E-GEOD-36819 | biostudies-arrayexpress

Sequential stage specific reporter line analysis (SSRLA) of 3 BAC transgenic mESC lines

Project description:FACS purified cells from differentiation day 14-15 cells from 3 BAC transgenic mESC lines: Hes::GFP (early), Nurr1::GFP (mid), and Pitx3::YFP (late) DA neuron development reporter lines

2012-07-12 | GSE37446 | GEO

Transcriptome of BAC-transgenic G1- and G0-APOL1 murine CD8+T-cells after ex-vivo stimulation.

Project description:BAC-transgenic G1-APOL1 mice ex-vivo stimulated CD8+T-cells show showed enrichment of canonical T-cell receptor (TCR) response pathways including calcium signaling.

2026-02-06 | GSE289030 | GEO

Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse.

2010-12-25 | GSE25607 | GEO

Transgenic expression of BEN, a member of TFII-I family, in various murine adipose tissues

Project description:Transgenic expression of BEN, a member of TFII-I family, in various murine adipose tissues

| PRJEB12245 | ENA

Gene expression analysis of embryonic photoreceptor precursor cells using BAC-Crx-EGFP transgenic mouse.

Project description:We generated a transgenic mouse line which express EGFP in the retina driven by the Crx promoter using BAC transgenesis. We sorted EGFP-positive photoreceptor precursors at E17.5 using FACS, and subsequently performed microarray analysis of the FACS-sorted cells. In order to clarify a molecular role of Crx in developing cone photoreceptor precursors, we investigated the expression profile of the BAC-Crx-EGFP-positive cells compared with that of the BAC-Crx-EGFP-negative cells at E17.5.

2010-12-25 | E-GEOD-25607 | biostudies-arrayexpress

Microarray of BAC transgenic Zfhx2 p.R1907K mutant mice (4 genomic copies) versus wild-types

Project description:A novel missense mutation has been identified in a human pain-insensitive family in the gene ZFHX2. We modelled this mutation in mice by changing the orthologous amino acid and carried out pain behaviour tests in mutant BAC transgenic mice. These showed a phenotype of hyposensitivity to noxious thermal stimuli. ZFHX2 has enriched expression in dorsal root ganglia neurons, and so we carried out microarray analyses in mutant vs wild type controls from lumbar DRG to see which genes were deregulated by the mutant ZFHX2 protein. To understand which genes are differentially expressed in lumbar DRG (L1-6) between Zfhx2 p.R1907K BAC transgenic mice (n=5, genomic BAC copy number of 4) and wild-type controls (n=7). Complementary ChIP-seq data for this study have also been deposited at ArrayExpress under accession number E-MTAB-5651 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5651 ).

2018-06-11 | E-MTAB-5650 | biostudies-arrayexpress

A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia (Bulk gene expression analysis of small intestinal glia cells in BAC-treated mice.)

Project description:Glial cells have been proposed as an endogenous source of progenitors for the treatment of neural deficits. However, the cellular and molecular mechanisms underpinning the neurogenic potential of certain populations of adult glial cells, are not known. Previous studies have indicated that chemical injury of the gut by benzalkonium chloride (BAC) of the gut can activate neuronal markers. To examine whether gut injury can induce in EGCs neurogenic programs similar to those expressed by early ENS progenitors in vivo, we performed bulk RNA-seq of enteric glia isolated from mouse guts 48 hours after BAC treatment. We isolated tdT+ cells (=EGCs) and tdT- cells (=non-glia cells) from the TM of control and BAC-treated Sox10CreERT2;Rosa26tdTomato mice and subjected the samples to bulk RNAseq analysis.

2023-08-11 | GSE240636 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data