Project description:To understand the gene expression dynamics during Flt3L induced Bone-marrow derived dendritic cell (BMDC) differentatiion we performed RNA-seq. Three distinct stages were chosen and included the initial bone marrow cells, the Common Myeloid Progenitor (CMP), and fully differentiated BMDC.
Project description:To investigate potential target genes of Zeb1 that were involved in regulation of antigen cross-presentation. We then performed gene expression profiling analysis using data obtained from RNA-seq from WT and Zeb1-deficient BMDC .
Project description:Hematopoietic progenitors from mouse bone marrow were differentiated into dendritic cells for 5 days with GM-CSF and IL-4. On day 6, MHCIIhi and MHCIIlo BMDC subpopulations were sorted to purify by flow cytometry. RNA was extracted and processed for microarray analysis.
Project description:Hematopoietic progenitors from mouse bone marrow were differentiated into dendritic cells for 5 days with GM-CSF and IL-4. On day 6, MHCIIhi and MHCIIlo BMDC subpopulations were sorted to purify by flow cytometry. RNA was extracted and processed for microarray analysis.
2013-12-22 | GSE52767 | GEO
Project description:RNA Seq analysis was conducted on mouse heart samples.
Project description:To investigate potential target genes of Zeb1 that were involved in regulation of antigen cross-presentation. We then performed gene expression profiling analysis using data obtained from RNA-seq from WT and Zeb1-deficient BMDC after HKLM-OVA stimulating for 4 hours.
Project description:To investigate potential target miRNA of Zeb1 that were involved in regulation of antigen cross-presentation. We then performed gene expression profiling analysis using data obtained from miRNA-seq from WT and Zeb1-deficient BMDC after HKLM-OVA stimulating for 4 hours.
Project description:Bone Marrow derived dendritic cells (BMDC) were cultured for 9 days. BMDC were treated with T cell exosomes for 6 hours and frozen for further analysis.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived WT or Bat3 KO BMDC transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived WT or Bat3 KO BMDC transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis
