Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1313630 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Grass carp is the most produced freshwater fish species in China. However, frequent outbreaks of bacterial diseases caused by Aeromonas ssp. have led to huge economic losses in grass carp farming. Various omics technologies have been used to study the response of grass carp to these pathogens. For instance, the transcriptional profile of the spleen from grass carp challenged with A. hydrophila, which revealed significant enrichment of gene clusters, including phagocytosis, complement system, cytokines, antigen processing and presentation, pattern recognition receptors, cell adhesion molecules, apoptosis, and antioxidant enzymes. Furthermore, a large number of differentially expressed genes related to inflammation were identified in the intestinal transcriptome of grass carp infected with A. hydrophila. However, the immune response of grass carp infected with A. veronii remains unresolved at the multi-omics level. In the present study, an intestinal infection model was established in grass carp using the isolated A. veronii strain EL07, and the differentially expressed genes and proteins in the intestinal and differentially expressed metabolites in serum were analyzed. The results may contribute to a better understanding of the pathogenesis of grass carp enteritis caused A. veronii.
2026-04-30 | GSE329120 | GEO
Project description:Few studies reported for obtaining the grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. Due that obvious cytopathic effect (CPE) in the present available cell lines is only caused by GCRV-I, but GCRV-II is the current popular and fatal strain in grass carp, GCRV-I and GCRV-II are used in cell lines and in grass carp, respectively. The in vitro studies in CIK cells revealed that gcPI4KB interacted with NS80 and VP3 of GCRV-I, and that gcPI4KB was recruited by NS80 for promoting the generation of GCRV VIBs. Since the negative regulatory role of gcPI4KB in GCRV infection was confirmed by in vitro data,we performed gene editing of gcPI4KB in grass carp. We found that PI4KB F0 crispants juvenile grass carp have obvious advantages in promoting growth and in resisting GCRV-II infection. Compared with uninfected WT grass carp, the uninfected PI4KB F0 crispants juvenile grass carp exhibit a higher expression level of many genes involved in growth- and development-related metabolic pathways such as the FoxO signaling pathway and insulin signaling pathway. Compared with WT grass carp without infection, PI4KB F0 crispants juvenile grass carp without infection or WT grass carp infected with GCRV-II, higher expression levels for many genes involved in metabolic diseases and viral infection were observed in the liver from PI4KB F0 crispants juvenile grass carp infected with GCRV-II. Altogether, the present study suggests the mechanism of gcPI4KB in facilitating GCRV replication, the signaling pathways regulated by gcPI4KB, and the possibility to obtain the grass carp resistant to hemorrhagic disease via gene editing of PI4KB.
2025-09-30 | GSE273923 | GEO
Project description:Hemorrhagic disease caused by grass carp reovirus (GCRV) infection is a major problem affecting the grass carp aquaculture industry. Therefore, inhibiting the spread of GCRV infection is of great economic significance. Herein, we sequenced five tissues (gill, liver, intestine, kidney, and muscle) from grass carp before and after GCRV infection using data-independent acquisition proteomic.
2024-10-20 | PXD056989 |
Project description:In the present study, an intestinal infection model was established in grass carp using the isolated A. veronii strain EL07, and the differentially expressed genes and proteins in the intestinal and differentially expressed metabolites in serum were analyzed. The results may contribute to a better understanding of the pathogenesis of grass carp enteritis caused A. veronii.
2026-04-27 | PXD077687 |
Project description:To elucidate the target genes of ArgR in Aeromonas veronii, we engineered an Aeromonas veronii strain that expresses the ArgR protein fused to a 3× FLAG tag, and FLAG antibodies were employed for the immunoprecipitation of DNA-protein complexes.
2024-09-15 | GSE277129 | GEO
Project description:The intestinal epithelial gene responses to Aeromonas veronii infection and the pathogenic mechanisms were investigated by comparative differential expression analysis
2023-07-28 | GSE222117 | GEO
Project description:The bacterium Aeromonas veronii is a co-pathogenic species that can negatively impact the health of both humans and aquatic animals. In this study, we used single-cell transcriptome analysis (scRNA-seq) to investigate the effects of infection with A. veronii on head kidney cells and the regulation of gene expression in the dark sleeper (Odontobutis potamophila). scRNA-seq was used to assess the effects of infection with A. veronii in O. potamophila B cells, endothelial cells, macrophages, and granulocytes, and differential enrichment analysis of gene expression in B cells and granulocytes was performed. The analyses revealed a significant increase in neutrophils and decrease in eosinophils in granulocytes infected with A. veronii. Activation of neutrophils enhanced ribosome biogenesis by up-regulating the expression of rps12 and rpl12 to fight against invading pathogens. Crucial pro-inflammatory mediators il1b, ighv1-4, and the major histocompatibility class II genes mhc2a and mhc2dab, which are involved in virulence processes, were up-regulated, suggesting that A. veronii activates an immune response that presents antigens and activates immunoglobulin receptors in B cells. These cellular immune responses triggered by infection with A. veronii enriched the available scRNA-seq data for teleosts, and these results are important for understanding the evolution of cellular immune defense and functional differentiation of head kidney cells.
2023-05-16 | GSE229275 | GEO
Project description:Largemouth bass (Micropterus salmoides) were challenged with Aeromonas veronii (Av), Aeromonas hydrophila (Ah), Flavobacterium columnare (Fc), and coinfections (AvFc, AhFc), alongside mock-challenged and pre-challenge controls. Gill and spleen tissues were sampled at 0h (pre-challenge baseline), 12h, and 24h post-challenge. RNA-Seq was performed on 161 samples (79 gill, 82 spleen; 6 biological replicates per treatment/timepoint group, 10 for pre-challenge). Three gill samples were excluded due to low mapping rates consistent with gDNA contamination (11B3G, 15J2G, 29R3G). Differential expression analysis was performed using DESeq2 across 72 pairwise contrasts per tissue.
2026-07-31 | GSE333434 | GEO
Project description:SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To further investigate the antiviral role SERPINA1 played in GCRV (Grass carp Reovirus) infection, a polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. Samples of hepatopancreas tissues from grass carp (n=3) before (healthy) and 8 days after the infection (post-infection) were selected. Grass carp were infected by intraperitoneal injection. The total tissue proteins were extracted according to the manufacturer’s instructions for cell lysate (Beyotime, Shanghai, China). The types and abundance of proteins bound to SERPINA1 before and after the infection were detected and analyzed using CoIP-MS.
2022-08-31 | PXD036356 | Pride
Project description:Grass carp (Ctenopharyngodon idellus), the world’s largest aquaculture fish species, exhibits superior growth in females compared to males. However, the lengthy sexual maturation period of four to five years poses a significant obstacle to the genetic reproduction and breeding of grass carp. Consequently, classical methods such as gonadogenesis or sex reversal through steroid treatment, employed for breeding all-female grass carp, demand considerable time and effort. In this study, we developed an super-fast breeding strategy for generating all-female grass carp in a total of half a year, using a surrogate production method. We first characterized grass carp female germline stem cells (GSCs) from genetic female juveniles at three months post-fertilization (mpf). The female GSCs with XX chromosomes were then transplanted into germ cell-depleted zebrafish larvae at five days post-fertilization (dpf). The transplanted grass carp XX germ cells underwent rapid spermatogenesis in the zebrafish recipient. At three months after transplantation, all zebrafish recipients had developed into males capable of producing the all-X sperm of the grass carp. By using these sperm to fertilize wildtype grass carp eggs, we successfully produced an all-female grass carp offspring. This groundbreaking achievement highlights the potential of surrogate production in the genetic breeding of valuable fish species, and opens a new avenue for advancing genetic breeding in aquaculture.
2025-03-01 | GSE261835 | GEO