Project description:The Purpose of this study is to screen for microRNAs regulated by hypoxia in neonatal rat cardiomyocytes. 3 biological replicates were perfomed for each each group, normoxia and hypoxia. One normoxic and one hypoxic sample was hybridized to each chip for a total of 6 2-color experiments. A dye swap for each oxygen treatment were performed.
Project description:Ten million neonatal rat ventricular cardiomyocytes were infected with adenovirus to overexpress NRF1 or LacZ (control). 48 hours after infection, cells were collected and lysed for TMT proteomic analysis.
Project description:Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later.
