Project description:Here we report the effect of two comparative transcriptomics experiments effect on EDDS producing biosynthetic gene cluster. The goals of this study are to compare RNA sequencing derived transcriptomes of Amycolatopsis japonica at 24 hours in zinc treated and control culture conditions in combination with control and zur regulator deleted strains, in order to target other genes for the overexpression of EDDS cluster.
Project description:We reported transcriptome profiles of rifamycin SV-produced strain Amycolatopsis mediterranei U32 in the Bennet medium with or without 80 mM nitrate in different cultivation stage. By comparative analysis of genome-wide gene expression in these conditions, we found that the mechanism of high production of rifamycin SV induced by nitrate could be elucidated.
Project description:We reported transcriptome profiles of rifamycin SV-produced strain Amycolatopsis mediterranei U32 in the Bennet medium with or without 80 mM nitrate in different cultivation stage. By comparative analysis of genome-wide gene expression in these conditions, we found that the mechanism of high production of rifamycin SV induced by nitrate could be elucidated. Examination of 2 time-course transcription in 2 different nitrogen source conditions
Project description:Amycolatopsis sp. BX17 is an actinobacterium isolated from milpa soils that antagonizes the phytopathogenic fungus Fusarium graminearum. Metabolites secreted by the actinobacterium cultured in medium without glucose inhibited 100% the mycelial growth of F. graminearum RH1, while in medium supplemented with 20 g/L of glucose inhibition was 65%. With the aim of studying how the metabolism of strain BX17 is modulated by glucose, as the main carbon source, media with 0 and 20 g/L glucose were selected to analyze the intracellular proteins by quantitative label-free proteomic analysis.
Project description:To identify carbohydrate-active enzymes in the secretome of strain A3 when L. japonica was the sole carbon source and key alginate lyases in the supernatant of fermentation broth.
