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Proteomic analysis reveals metabolic versatility of Amycolatopsis sp. BX17: a strain native from milpa agroecosystem soil

ABSTRACT: Amycolatopsis sp. BX17 is an actinobacterium isolated from milpa soils that antagonizes the phytopathogenic fungus Fusarium graminearum. Metabolites secreted by the actinobacterium cultured in medium without glucose inhibited 100% the mycelial growth of F. graminearum RH1, while in medium supplemented with 20 g/L of glucose inhibition was 65%. With the aim of studying how the metabolism of strain BX17 is modulated by glucose, as the main carbon source, media with 0 and 20 g/L glucose were selected to analyze the intracellular proteins by quantitative label-free proteomic analysis.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Amycolatopsis

TISSUE(S): Cell Culture

SUBMITTER: José Ángel Huerta-Ocampo

LAB HEAD: José Ángel Huerta-Ocampo

PROVIDER: PXD028644 | Pride | 2022-02-14

REPOSITORIES: Pride

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Proteomic analysis reveals the metabolic versatility of Amycolatopsis sp. BX17: A strain native from milpa agroecosystem soil.

Palafox-Félix Michel M Huerta-Ocampo José Ángel JÁ Hernández-Ortíz Magdalena M Encarnación-Guevara Sergio S Vázquez-Moreno Luz L Guzmán-Partida Ana María AM Cabrera Rosina R

Journal of proteomics 20211216

Amycolatopsis sp. BX17 is an actinobacterium isolated from milpa soils, which antagonizes the phytopathogenic fungus Fusarium graminearum. Metabolites secreted by the actinobacterium cultured in glucose-free medium inhibited 100% of the mycelial growth of F. graminearum RH1, while the inhibition rate was 65% in medium supplemented with 20 g/L glucose. With the aim of studying how the metabolism of strain BX17 is modulated by glucose as the main carbon source, media with 0 and 20 g/L glucose were ...[more]

PMID: 34922014

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