Medicago sativa
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA13214 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR003981.fastq.gz | Fastqsanger.gz | |||
| DRR003982.fastq.gz | Fastqsanger.gz | |||
| DRR003983.fastq.gz | Fastqsanger.gz | |||
| DRR003984.fastq.gz | Fastqsanger.gz | |||
| SRR094951.fastq.gz | Fastqsanger.gz |
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Project description:Alfalfa (Medicago sativa) is the most widely grown and most important forage crop in the world. However, alfalfa is susceptible to waterlogging stress, which is the major constraint for its cultivation area and crop production. So far, the molecular mechanism of alfalfa response to the waterlogging is largely unknown. Here, comparative transcriptome combined with proteomic analyses of two cultivars (M12, tolerant; M25, sensitive) of alfalfa showing contrasting tolerance to waterlogging were performed to understand the mechanism of alfalfa in response to waterlogging stress. Totally, 748 (581 up- and 167 down-regulated) genes were differentially expressed in leaves of waterlogging-stressed alfalfa compared with the control (M12_W vs M12_CK), whereas 1193 (740 up- and 453 down-regulated) differentially abundant transcripts (DATs) were detected in the leaves of waterlogging-stressed plants in comparison with the control plants (M25_W vs M25_CK). Furthermore, a total of 187 (122 up- and 65 down-regulated) and 190 (105 up- and 85 down-regulated) differentially abundant proteins (DAPs) were identified via iTRAQ method in M12_W vs M12_CK and M25_W vs M25_CK comparison, respectively. Compared dataset analysis of proteomics and transcriptomics revealed that 27 and 8 genes displayed jointly up-regulated or down-regulated expression profiles at both mRNA and protein levels in M12_W vs M12_CK comparison, whereas 30 and 27 genes were found to be co-up-regulated or co-down-regulated in M25_W vs M25_CK comparison, respectively. The strongly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for co-up-regulated genes at mRNA and protein levels in M12_W vs M12_CK comparison were ‘Amino sugar and nucleotide sugar metabolism’, ‘Arginine and proline metabolism’ and ‘Starch and sucrose metabolism’, whereas co-up-regulated protein-related pathways including ‘Arginine and proline metabolism’ and ‘Valine, leucine and isoleucine degradation’ were largely enriched in M25_W vs M25_CK comparison. Importantly, the identified genes related to beta-amylase, Ethylene response Factor (ERF), Calcineurin B-like (CBL) interacting protein kinases (CIPKs), Glutathione peroxidase (GPX) and Glutathione-S-transferase (GST) may play key roles in conferring alfalfa tolerance to waterlgging stress. The present study may contribute to our understanding the molecular mechanism underlying the responses of alfalfa to waterlogging stress, and also provide important clues for further study and in-depth characterization of waterlogging-resistance breeding candidate genes in alfalfa.
2019-11-11 | PXD013025 | Pride
Project description:Alfalfa cultivars (Medicago sativa L.) is an imporant forage crop and widely cultivated in China and many countries. Raw sequence reads
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Project description:<p>Common vetch (Vicia sativa L.) is an important annual leguminous forage crop commonly used for green manure, fodder, and soil improvement. It is widely cultivated as a green manure and forage crop in Yunnan Province during winter and spring. However, the dry conditions and minimal rainfall during these seasons greatly limit common vetch growth. Therefore, screening for drought-tolerant, high-yielding common vetch varieties is a critical objective in breeding programs.</p>
2026-01-31 | MTBLS13368 | MetaboLights
Project description:Medicago sativa L. is the most extensively cultivated and high biomass forage. Selenium is an essential microscale mineral for humans and animals and is a beneficial element for plants, which could increase yields under low concentration and have a poisoning effect under high concentration. LncRNA is involved in diverse biological processes such as flowering, photomorphogenesis, vernalization and stress response in the plants. To study the possible role of lncRNAs in Se accumulation and detoxification underlying alfalfa, we identified and analyzed lncRNAs in alfalfa leaves under 0 mg L-1, 100 mg L-1, 500 mg L-1 based on the degradome deep sequencing. We identified a total of 64,684 novel lncRNAs, from which 3155 were differentially expressed lncRNAs (DELs). The expression patterns of some lncRNAs can be significantly differentially expressed between high and low concentrations. The results of GO and KEGG analysis suggested that sulfate transporter and ABC transporter might be a contributor to Se accumulation in alfalfa. The jasmonic acid (JA) promoted by lipoxygenase and allene oxide synthase may play an important role in Se resistance. The high expression of some key genes of selenocompound metabolism may be beneficial to remove excess Se. The phenylpropanoid, flavonoid, isoflavonoid, and anthocyanidin biosynthesis and autophagy may alleviate the Se toxicity by scavenging reactive oxygen. The expression patterns of nine lncRNAs and their protein-coding genes were validated by qRT-PCR. The same expression pattern was observed between most lncRNAs and their target genes. This study has excavated potential lncRNAs and will give new insight and rich genetic resources to Se-enriched breeding.
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Project description:Forage crop legumes Transcriptome
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Project description:Alfalfa (Medicago sativa L.) is a forage legume with significant agricultural value worldwide. MicroRNAs (miRNAs) are key components of post-transcriptional gene regulation and essentially control almost all aspect of plant growth and development. Although miRNAs have been reported from alfalfa but their expression profiles in different tissues and novel miRNAs as well as their targets have not been confirmed in this plant species. Therefore, we sequenced small RNAs in whole plantlets, shoots and roots of three different alfalfa genotypes (Altet-4, NECS-141 and NF08ALF06) to identify tissue-specific profiles. After comprehensive analysis using bioinformatics methods, we have identified 100 miRNA families, of which 21 belongs to the highly conserved families whereas the remaining 79 families are conserved between M. truncatula and M. sativa. The profiles of the six highly expressed conserved miRNA families (miR156, 159, 166, 319, 396, 398,) were relatively similar between the plantlets, roots and shoots of three genotypes. Contrastingly, the differenecs were robust between shoots and roots for miR160 and miR408 levels, which were low in roots compared to shoots. The study also has identified 17 novel miRNAs that also differed in their abundanecs between tissues of the alfalfa genotypes. Additionally, we have generated and analyzed the degradome libraries from three alfalfa genotypes that has confirmed 69 genes as targets for 31 miRNA families in alfalfa. The identification of conserved and novel miRNAs as well as their targets in different tissues of three genotypes not only enhanced our understanding of miRNA-mediated gene regulation in alfalfa but could also be useful for practical applications in alfalfa as well as related legume species.
2019-01-15 | GSE119460 | GEO
Project description:G-protein subunits were characterized from Medicago sativa (alfalfa) seedlings. Crude membranes and GTP-Sepharose-purified fractions were electrophoresed on SDS/polyacrylamide gels and analysed by Western blotting with 9193 (anti-alpha common) and AS/7 (anti-alpha t, anti-alpha i1 and anti-alpha i2) polyclonal antibodies. These procedures led to the identification of a specific polypeptide band of about 43 kDa. Another polypeptide reacting with the SW/1 (anti-beta) antibody, of about 37 kDa, was also detected. The 43 kDa polypeptide bound specifically [alpha-32P]GTP by a photoaffinity reaction and was ADP-ribosylated by activated cholera toxin, but not by pertussis toxin. Irradiation of etiolated Medicago sativa protoplast preparations at 660 nm for 1 min produced a maximal increase in the guanosine 5'-[gamma-thio]triphosphate (GTP[35S])-binding rate. After this period of irradiation, the binding rate tended to decrease. The effect of a red-light (660 nm) pulse on the binding rate was reversed when it was immediately followed by a period of far-red (> 730 nm) illumination. These results may suggest that activation of GTP[S]-binding rate was a consequence of conversion of phytochrome Pr into the Ptr form.
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Project description:In this study, proteomics was used to sequence the salt stress treatment group and the control group of Medicago sativa and Medicago truncatula. The aim was to discover the kegg pathway of the two alfalfa varieties under salt stress, which was of great significance to the exploration of the salt tolerance mechanism of alfalfa.
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Project description:An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.
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