Project description:Exoproteomes from the yeast Vishniacozyma dimennae blapla were analyzed in order to identify putative polyesterases responsible for its ability to hydrolyze various biodegradable plastics. To this end, the yeast was grown in a mineral medium and in the presence of different polyesters, like poly(caprolactone), poly(lactic acid), poly(ethylene succinate), poly(butylene succinate) and poly(butylene adipate-co-terephthalate), as well as with pyruvate and glucose as controls. The ultimate goal was to overexpress putative detected polyesterases to find the one responsible for the polyester-hydrolyzing phenotype.
Project description:Based on the genome sequencing and gene annotation, we analyzed the alginate lyases and other genes related to alginate metabolism of strain Vibrio sp. C42, with the highest alginate-degrading activity. Combined with proteome measurement, we proposed its alginate metabolic pathways.
Project description:Leishmania donovani WHO reference strain MHOM/IN/80/DD8 and Leptomonas seymouri isolates Ld 2001 and Ld39 were used for proteome analysis which were originally isolated from clinical cases of kala azar patients with different inherent antimonial sensitivities. Ld 2001 was Sb-S and Ld 39 was Sb-R. The genome sequencing of these isolates had confirmed co-infection with Leptomonas.