Project description:Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct microarray analysis on broccoli florets of N6-benzylaminopurine treated and ipt-transgenic broccoli that harbor a senescence-associated-gene promoter triggering isopentenyltransferase gene expression during postharvest storage. Analysis used RNA of Green King inbred line 104 as control sample for comparison to the experimental samples of ipt-transgenic line 102, 103 and parental line Green King as well as 10 ppm BA treated Green King at harvest and after postharvest storage at 25 centigrade in the dark for 4 days.
Project description:Both exogenously supplied and transgenic induced cytokinin production can effectively delay senescence of broccoli florets during postharvest storage. However, a substantial comparison between the mechanisms of these two treatments on delaying broccoli florets senescence was absent. Here, we conduct microarray analysis on broccoli florets of N6-benzylaminopurine treated and ipt-transgenic broccoli that harbor a senescence-associated-gene promoter triggering isopentenyltransferase gene expression during postharvest storage.
Project description:Melatonin is a well-known agent that plays multiple roles in animals. Its possible function in plants is less clear. In the present study, we tested the effect of melatonin (N-acetyl-5-methoxytryptamine) on soybean growth and development. Both spraying of leaves and seed-coating with melatonin significantly promoted soybean growth as judged from leaf size and plant height. This enhancement was also observed in soybean production and their fatty acid content. Melatonin increased pod number, seed number and seed weight. However, the 100-seed weight was not influenced by melatonin application. Melatonin also improved soybean tolerance to salt and drought stresses. Transcriptome analysis revealed that melatonin up-regulated the expression of many genes and alleviated the inhibitory effects of salt stress on gene expressions. Further detailed analysis of the affected pathways documents that melatonin likely achieved its promotional roles in soybean through enhancement of genes involved in cell division, photosynthesis, carbohydrate metabolism, fatty acid biosynthesis and ascorbate metabolism. Our results demonstrate that melatonin has significant potential for improving of soybean growth and seed production. Further study should uncover more about the molecular mechanisms of melatoninM-bM-^@M-^Ys function in soybeans and other crops. Four different treatments were chosen, water, salt, 100M-BM-5M melatonin and salt plus 100M-BM-5M melatonin. The comparison of salt/melatonin-treated sample versus water-treated sample reveals salt or melatonin induced transcriptome changes. The comparison of melatonin plus salt treated sample versus salt-treated sample reveals melatonin induced changes when salt exists.
Project description:The postharvest senescence processes of citrus fruits were analyzed transcriptomic. The present study was aimed to: further uncover the rind-flesh communication of hesperidium; characterize the differential storage behaviors of different citrus varieties; reveal the important changes during storing process; and demonstrate the specific non-climacteric characteristics of citrus fruits.
Project description:Melatonin is a well-known agent that plays multiple roles in animals. Its possible function in plants is less clear. In the present study, we tested the effect of melatonin (N-acetyl-5-methoxytryptamine) on soybean growth and development. Both spraying of leaves and seed-coating with melatonin significantly promoted soybean growth as judged from leaf size and plant height. This enhancement was also observed in soybean production and their fatty acid content. Melatonin increased pod number, seed number and seed weight. However, the 100-seed weight was not influenced by melatonin application. Melatonin also improved soybean tolerance to salt and drought stresses. Transcriptome analysis revealed that melatonin up-regulated the expression of many genes and alleviated the inhibitory effects of salt stress on gene expressions. Further detailed analysis of the affected pathways documents that melatonin likely achieved its promotional roles in soybean through enhancement of genes involved in cell division, photosynthesis, carbohydrate metabolism, fatty acid biosynthesis and ascorbate metabolism. Our results demonstrate that melatonin has significant potential for improving of soybean growth and seed production. Further study should uncover more about the molecular mechanisms of melatonin’s function in soybeans and other crops.
Project description:Senescence (or aging) is the final stage of plant development; senescence involves a complex programmed process that is closely related to biotic and abiotic stresses and is regulated by fine-tuned molecular mechanisms. Postharvest vegetative organs undergo a series of physiological and biochemical changes during senescence. However, if postharvest plant organs were not scientifically processed or cured, there would be a great loss in their commercial value during curing or storage. Thus, studying the molecular mechanisms underlying senescence of plant organs will enhance understanding of such a fundamental biological process and might provide new insights into controlling senescence and improving commercial value during curing or storage. However, few studies have focused specifically on the molecular mechanism of leaf senescence affecting tobacco quality at the proteomic level during the yellowing stage. The main objectives of this work were to determine the protein profile changes associated with different curing stages and to establish links between protein profile changes and senescence physiology to provide a more in-depth understanding of the mechanism of senescence in postharvest tobacco leaves under oxidation, heat/high temperature, dehydration and starvation stresses at the proteomic level. This study provides new insights into the postharvest physiology and molecular mechanism of senescence at the proteomic level in postharvest leaves of tobacco, a model solanaceous plant, in response to curing and senescence during the yellowing stage.
2020-01-06 | PXD016941 |
Project description:Integrative metabolome and transcriptome analyses reveal the molecular mechanism of thermal stress response in tobacco leaves
Project description:Exposure to UV-C irradiance effectively maintains fruit quality, however the mechanisms by which it ensures tomato postharvest quality remain unclear. We conducted a comprehensive analysis of the transcriptome, volatilome, DNA methylome, and RNA methylome of tomato fruit in order to construct a UV-C regulatory network. UV-C was found to induce the expression of hormones-related, flavonoid, and resistance genes, to delay senescence, and to promote the expression of sugar and acid metabolism genes, leading to the synthesis of soluble sugars and degradation of citric acid in a manner that maintains fruit taste characteristics. Volatilome results demonstrated that UV-C enhanced the accumulation of 3-methyl-2-butenal, β-damascenone, and 6-methyl-5-heptene-2-one by regulating genes associated with the metabolism of these volatile organic compounds. Additionally, UV-C contributed to the retention of accessible chromatin regions (ACRs), with genes associated with plant hormones showing variation in expression corresponding to chromatin accessibility. UV-C also inhibited the decrease in DNA methylation during ripening, with dynamic changes in DNA methylation and RNA methylation of genes related to hormones, flavonoids, texture, and resistance, ultimately delaying fruit senescence. This study is the first to comprehensively elucidate the regulatory role of UV-C on tomato postharvest ripening and as such provides valuable insights into epigenetic modifications which could represent important tools for future quality-based breeding programs.
Project description:The postharvest senescence processes of citrus fruits were analyzed transcriptomic. The present study was aimed to: further uncover the rind-flesh communication of hesperidium; characterize the differential storage behaviors of different citrus varieties; reveal the important changes during storing process; and demonstrate the specific non-climacteric characteristics of citrus fruits. We chose four major table fruit varieties of citrus: satsuma mandarin (Citrus unshiu Marc) (M), ponkan (Citrus reticulata Blanco) (K), newhall navel orange (Citrus sinensis L. Osbeck) (O) and shatian pummelo (Citrus grandis Osbeck) (P). They were sampled every 10 days during 50 DAH (days after harvest), almost covering the commercial storage period of loose-skin citrus.