Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA133005 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Detection of aberrant DNA methylation in undifferentiated and differentiated mouse ES cells compared to normal tissues
| PRJNA130375 | ENA
Project description:Detection of aberrant DNA methylation in undifferentiated and differentiated mouse ES cells compared to normal tissues
| PRJNA237315 | ENA
Project description:In this study, we compared gene expression profiles of c-kit+Sca-1+ cells generated in vitro from mouse ESCs using static and bioreactor-based cultures with native HSCs isolated from mouse fetal liver (FL) or bone marrow (BM). total RNA obtained from ckit+Sca-1+ cells isolated from mouse embryonic stem cells (ES) differentiated for 7 days in Static, Spinner flask and Synthecon compared to RNA isolated from ckit+Sca-1+ cells from mouse bone marrow, mouse fetal liver and mouse ES cells undifferentiated.
2012-11-15 | E-GEOD-27116 | biostudies-arrayexpress
Project description:Detection of aberrant DNA methylation in acute leukemia samples compared to normal human monocytes
| PRJNA118779 | ENA
Project description:Detection of aberrant DNA methylation in colorectal carcinoma samples compared to normal human colon
| PRJNA118783 | ENA
Project description:Detection of aberrant DNA methylation in AML cell lines compared to normal human monocytes
| PRJNA118727 | ENA
Project description:Primary pediatric Ewing sarcoma (ES), one uncharacterized sarcoma as well as primary and well established ES cell lines were compared to probes of different normal tissues 8 Ewing sarcoma patient samples (MuET-x), 3 primary ES cell lines (SB-KMS-y), 3 well established ES cell lines (A673, SK-N-MC, RD-ES) and 22 normal tissues (PBMC, spleen, thymus, stomach, ...., uterus, fetal brain, fetal liver) were analyzed.
2013-05-01 | E-GEOD-45544 | biostudies-arrayexpress
Project description:Detection of aberrant DNA methylation in acute myeloid leukemia samples compared to normal human monocytes
| PRJNA438533 | ENA
Project description:Publication title: Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver; To investigate the role of DNA methylation during human development, we developed Methyl-seq, a method that assays DNA methylation at more than 90,000 regions throughout the genome. Performing Methyl-seq on human embryonic stem cells (hESCs), their derivatives and human tissues allowed us to identify several trends during hESC and in vivo liver differentiation. First, differentiation results in DNA methylation changes at a minimal number of assayed regions, both in vitro and in vivo (2-11%). Second, in vitro hESC differentiation is characterized by both de novo methylation and demethylation, whereas in vivo fetal liver development is characterized predominantly by demethylation. Third, hESC differentiation is uniquely characterized by methylation changes specifically at H3K27me3-occupied regions, bivalent domains and low-density CpG promoters (LCPs) suggesting that these regions are more likely to be involved in transcriptional regulation during hESC differentiation. Although both H3K27me3-occupied domains and LCPs are also regions of high variability in DNA methylation state during human liver development, these regions become highly unmethylated, which is a distinct trend from that observed in hESCs. Taken together, our results indicate that hESC differentiation has a unique DNA methylation signature that may not be indicative of in vivo differentiation. Experiment Overall Design: lumina gene expression beadchips of human ES cell lines, ES-derived cells, and normal liver (15 samples). High-throughput sequencing of ES cell lines, ES-derived cells, and fetal and normal livers (17 samples). Raw data: SRA008154 http://www.ncbi.nlm.nih.gov/sites/entrez?db=sra&cmd=search&term=SRA008154
2009-08-29 | E-GEOD-14966 | biostudies-arrayexpress
Project description:Blood and endothelial cells arise from hemangiogenic progenitors that are specified from FLK1-expressing mesoderm by the transcription factor ETV2. FLK1 mesoderm also contributes to other tissues, including vascular smooth muscle (VSM) and cardiomyocytes. However, the developmental process of FLK1 mesoderm generation and its derivatives and the lineage relationship among FLK1 mesoderm derivatives these tissues remain obscure. Recent single cell RNA-sequencing (scRNA-seq) studies of early stages of embryogenesis embryos, or in vitro differentiated human embryonic stem (ES) cells have differentiation provided unprecedented information on the spatiotemporal resolution of cells in embryogenesis. Nonetheless, these snapshots still nonetheless offer insufficient information on dynamic developmental processes due to inadvertently missing intermediate states and unavoidable batch effects. Here we performed scRNA-seq of mouse ES cells in asynchronous embryoid bodies (EBs), in vitro differentiated embryonic stem (ES) cells containing undifferentiated ES cells and its differentiated hemangiogenic progeny, as well as yolk sacs, the first hematopoietic extraembryonic tissue in developing embryo that contains hemangiogenic and VSM lineages. We captured a continuous developmental process from undifferentiated pluripotent cells to FLK1 mesoderm-derived tissues involved in hemangiogenesis. This continuous transcriptome map will benefit both basic and applied studies of mesoderm and its derivatives.
2019-11-25 | GSE130146 | GEO