Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA133213 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE24002: Gis2 overexpression versus control cells GSE24003: Gis2 affinity isolations GSE24004: Gis2 mutant versus wild-type cells Refer to individual Series
2010-12-22 | E-GEOD-24005 | biostudies-arrayexpress
Project description:To identify RNAs specifically associated with Gis2p, cells expressing TAP-tagged Gis2 or the wild-type strain BY4741 (mock control) were grown to mid-log phase in rich medium and harvested by centrifugation. RNA affinity isolations were essentially performed as described (Gerber et al. 2004 PLoS Biol.; see associated protocol 526). In brief, TAP-tagged protein were captured from cell extracts with IgG coupled agarose beads (Sigma) and released by incubation with a site-specific protease (AcTEV, Sigma). from the extract (input) and from the affinity isolates was purified with the RNeasy Mini/ Micro Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. Set of arrays that are part of repeated experiments Genotype: TAP-tagged Gis2 expressing or wild-type strain BY4741 (wt/gis2)
2010-12-22 | GSE24003 | GEO
Project description:Gis2 overexpression versus control cells
| PRJNA133211 | ENA
Project description:To identify RNAs specifically associated with Gis2p, cells expressing TAP-tagged Gis2 or the wild-type strain BY4741 (mock control) were grown to mid-log phase in rich medium and harvested by centrifugation. RNA affinity isolations were essentially performed as described (Gerber et al. 2004 PLoS Biol.; see associated protocol 526). In brief, TAP-tagged protein were captured from cell extracts with IgG coupled agarose beads (Sigma) and released by incubation with a site-specific protease (AcTEV, Sigma). from the extract (input) and from the affinity isolates was purified with the RNeasy Mini/ Micro Kit (Qiagen). RNAs were reverse transcribed using a mixture of oligo-dT and random nonamer oligos in the presence of amino-allyl dUTP/dNTP mixture. cDNAs were fluorescently labeled and hybridized on yeast oligo microarrays over night at 42 degrees in formamide-based hybridization buffer. Set of arrays that are part of repeated experiments Genotype: TAP-tagged Gis2 expressing or wild-type strain BY4741 (wt/gis2) Biological Replicate
2010-12-22 | E-GEOD-24003 | biostudies-arrayexpress
Project description:Delayed phosphate starvation response in cells with abundant high-affinity transporters
| PRJNA147607 | ENA
Project description:Affinity purification of ribosomes and associated RNAs from stress-treated cells
| PRJNA113893 | ENA