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Pseudomonas putida KT2440

ABSTRACT: Expression data from Pseudomonas putida KT2440 (RNA-Seq data)

PROVIDER: PRJNA133713 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR066731.fastq.gz	Fastqsanger.gz
	SRR066732.fastq.gz	Fastqsanger.gz

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Understanding Isoprenol effect on Pseudomonas putida KT2440 grown on a minimal medium supplemented with glucose

Project description:Pseudomonas putida KT2440 (KT2440) has been established as an industrially relevant chassis for the production of the jet-fuel precursor isoprenol. However, the wild type KT2440 strain consumed isoprenol as sole carbon source and its growth is inhibited by isoprenol, which is an impediment to high titer, rate, yield production. In this study, we investigated genes responsible for isoprenol catabolism and the stress responses of Pseudomonas putida via RNA-seq.

2026-04-20 | GSE281392 | GEO

Generation of Ionic Liquid Tolerant Pseudomonas putida KT2440 Strains via Adaptive Laboratory Evolution

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Transcriptome of Pseudomonas putida KT2440 in response to selenite

Project description:The bacterium Pseudomonas putida KT2440 has the ability to reduce selenite forming nanoparticles of elemental selenium. This is the transcriptome of the organism when cultured in the presence of selenite.

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Evaluation of Pseudomonas putida KT2440 gene expression in the Arabidopsis thaliana rhizosphere

Project description:Gene expression patterns of the plant colonizing bacterium,Pseudomonas putida KT2440 were evaluated as a function of growth in the Arabidopsis thaliana rhizosphere. Gene expression in rhizosphere grown P. putida cells was compared to gene expression in non-rhizosphere grown cells. Keywords: Gene expression

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Transcriptomic analysis of (S)-1,3-butanediol degradation pathway in Pseudomonas putida KT2440

Project description:(S)-1,3-butanediol [(S)-1,3-BDO] is a chiral diol and stereoisomer of (R)-1,3-BDO with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (S)-1,3-BDO and (S)-3-hydroxybutyrate [(S)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Differential gene expression analysis was performed to identify genes specifically induced during (S)-1,3-BDO and (S)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.

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Characterization of DkaiC mutants of Pseudomonas putida KT2440/2 and Pseudomonas protegens CHA0

Project description:KaiC is the central cog of the circadian clock in Cyanobacteria. Close homologs of this protein are widespread among bacteria not known to have a circadian physiology. The function, interaction network, and mechanism of action of these KaiC homologs are still largely unknown. Here, we focus on KaiC homologs found in environmental Pseudomonas species. We characterize experimentally the only KaiC homolog present in Pseudomonas putida KT2440 and Pseudomonas protegens CHA0. Through phenotypic assays and transcriptomics, we show that KaiC is involved in osmotic and oxidative stress resistance in P. putida and in biofilm production in both P. putida and P. protegens.

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Project description:(R)-1,3-butanediol [(R)-1,3-BDO] is a chiral diol with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (R)-1,3-BDO and (R)-3-hydroxybutyrate [(R)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Gluconate was used as a reference carbon source. Differential gene expression analysis was performed to identify genes specifically induced during (R)-1,3-BDO and (R)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.

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