Project description:Transcription profiling by array of bronchial epithelial cells from severe asthmatic, mild-moderate asthmatic and non-asthmatic patients
Project description:Airway inflammation is the hallmark of asthma and suggests a dysregulation of homeostatic mechanisms. MicroRNAs (miRNAs) are key regulators of gene expression, necessary for the proper function of cellular processes. We used miRNA microarrays to compare the profiles of human bronchial epithelial cells from healthy and asthmatic donors Human bronchial epithelial cells were isolated from healthy and asthmatic donors. RNA was extracted and miRNA expression was analyzed on Affymetrix miRNA microarrays. We sought to utilize miRNA expression as a tool for understanding underlying biological differences in BECs from healthy and asthmatic donors.
Project description:Airway inflammation is the hallmark of asthma and suggests a dysregulation of homeostatic mechanisms. MicroRNAs (miRNAs) are key regulators of gene expression, necessary for the proper function of cellular processes. We used miRNA microarrays to compare the profiles of human bronchial epithelial cells from healthy and asthmatic donors
Project description:Real-time quantitative PCR analysis of microRNA expression in Primary human Bronchial Epithelial cells (PBECs) from healthy or asthmatic subjects
Project description:Transcription profiling by array of cultured bronchial epithelial cells from patients with asthma and healthy individuals after infection with human rhinovirus
Project description:Interleukin (IL)-17 plays an important and protective role in host defence and has been demonstrated to orchestrate airway inflammation by cooperating with and inducing proinflammatory cytokines. Mircoarrays were used to identify immediate-early/ primary response IL-17A-dependent gene transcripts in primary human bronchial ASM cells from mild asthmatic and healthy individuals. To evaluate IL-17A-inducible gene transcripts, primary human bronchial ASM cells from 3 mild asthmatic and 3 healthy donors were treated for 2h with IL-17 [10ng/ml] and were probed with the Affymetrix GeneChip array. The 2h time point was carefully chosen in order to identify primary response gene targets and to avoid confounding autocrine mechanisms mediating indirect, or late-phase gene expression responses. Non-stimulated ASM cells from the same patients were used as controls.
Project description:The goal of this study is to compare the base-line microRNA expression of asthmatic and healthy PBECs. PBECs were grown in-vitro up to passage 2 before harvest. RT-qPCR based microRNA array expression profiling. PBECs were isolated from 5 healthy and 5 asthmatic donors. Total cellular RNA from each sample was processed and analyzed independently.
