Project description:Leaves of the potatoes grown for 3 and 8 weeks at NT and HT were harvested for total RNA extraction. Total RNA was extracted from 100 mg of ground leaves using the Trizol reagent (MRC) following the manufacturer’s instructions. Five micro gram of each RNA sample were generated for a strand-specific library as previously described. The library was containing inserts of 150-200 bp. For RNA sequencing, paired-end 150 nt sequencing was performed using a HiSeq 4000 platform (Illumina, USA) at Microgen (Korea).
Project description:Potato wild relatives (Solanum section Petota) are a source of genetic diversity for improving traits in modern cultivars (S. tuberosum) to meet climate challenges. Potatoes are susceptible to multiple abiotic and biotic stresses and have undergone constant improvement through breeding programs worldwide. The allotetraploid S. acaule Bitter has been used to introgress cold tolerance into potato breeding germplasm. The cold challenged transcriptome of S. acaule was compared with that of autotetraploid S. tuberosum cv. Atlantic, and was found to have fewer differentially expressed genes than the latter. Specifically, subgenome 1 has less downregulated alleles compared to subgenome 2 and S. tuberosum.
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
Project description:orf137 and orf320 are Candidates for Mitochondrial Genes Associated with W/alpha and T/beta-type Cytoplasmic Male Sterility in Potatoes
