Project description:Transcriptional profiling of S. thermophilus LMD-9 wild-type compared to the isogenic mutant strain CB052 (deletion of mecA, STER_0216) for the identification of the MecA regulon. Cells were grown in THB medium supplemented with glucose 1% (THBG) and sampled at OD600 = 0.4 for mRNA extraction wild-type vs knockout, 2 WT biological replicates (one used as technical replicate for a dye swap), 2 KO biological replicates (one used as technical replicate for a dye swap)
Project description:Interactome of overexpressed, HA-tagged AIFM1 in HEK293T AIFM1 KO background vs control (3797, 3593), Interactome of overexpressed, HA-tagged AIFM1 variants in HEK293T AIFM1 KO background vs control (3859), Comparison of whole cell extract after gelfiltration of HEK293T AIFM1 KO cell line vs WT