Project description:PC3GLI1ER and PC3ER were established from human prostate cancer cell line PC3. PC3GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, PC3ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by array
Project description:PC3GLI1ER and PC3ER were established from human prostate cancer cell line PC3. PC3GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, PC3ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by array
Project description:To identify which lncRNAs are differentially expressed in prostate cancer, total RNA from prostate cancer cell line, PC3, and normal epithelial prostatic cells were screened using NCode (Life Technologies) . The NCode microarray is designed to interrogate 12,784 lncRNAs and 25,409 mRNA target protein-coding genes. Gene expression in prostate epithelial cells and PC3 was measured. Three independent experiments (biological replicates) were performed.
Project description:To identify which lncRNAs are differentially expressed in prostate cancer, total RNA from prostate cancer cell line, PC3, and normal epithelial prostatic cells were screened using NCode (Life Technologies) . The NCode microarray is designed to interrogate 12,784 lncRNAs and 25,409 mRNA target protein-coding genes.
