Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA142447 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:miRNAs deregulated by LMP2A in nasopharyngeal cancer
| PRJNA142445 | ENA
Project description:Our data shows that LMP2A promotes a more aggressive tumor phenotype. To evaluate the miRNAs deregulated by LMP2A, RNA from the LMP2A cells and xenografted tumors were subjected to miRNA microarray.
2023-02-04 | GSE25682 | GEO
Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
2019-07-31 | GSE114179 | GEO
Project description:Latent infection with Epstein-Barr virus (EBV) is recognised as a factor in the pathogenesis of nasopharyngeal carcinoma (NPC). We found that EBV encoded Latent membrane protein 2A (LMP2A) enhances lipid accumulation significantly in NPC cells. We used microarrays to identify differential genes regulated by LMP2A in NPC cell lines.
2018-08-21 | GSE118807 | GEO
Project description:Our data shows that LMP2A promotes a more aggressive tumor phenotype. To evaluate the gene expression deregulated by LMP2A, RNA from the LMP2A cells and xenografted tumors were subjected to gene expression array.
2023-02-04 | GSE25683 | GEO
Project description: Not available
| S-EPMC2531291 | biostudies-literature
Project description:Differentially expressed genes in radioresistant nasopharyngeal cancer cells
| PRJNA100045 | ENA
Project description:Stem-like cells of nasopharyngeal cancer were enriched by 3 different methods: 1) Side population (SP) assay, 2) LMP2A over-expression and 3) spheroid culture. SP cells and LMP2A over-expressed cells were inoculated into immunocompromised mice for xenograft tumors. Transcriptomes of all 5 of these in vitro and in vivo generated NPC stem-like cells were then analyzed and compared to their counterparts by microarray. All samples were analyzed in the Agilent platform except the SP cells and their counterpart (analyzed in the Affymetrix platform).
2023-02-04 | GSE36125 | GEO
Project description:Stem-like cells of nasopharyngeal cancer were enriched by 3 different methods: 1) Side population (SP) assay, 2) LMP2A over-expression and 3) spheroid culture. SP cells and LMP2A over-expressed cells were inoculated into immunocompromised mice for xenograft tumors. Transcriptomes of all 5 of these in vitro and in vivo generated NPC stem-like cells were then analyzed and compared to their counterparts by microarray. All samples were analyzed in the Agilent platform except the SP cells and their counterpart (analyzed in the Affymetrix platform).
2023-02-04 | GSE36124 | GEO
Project description:We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We report that the majority of miRNA genes encode mature isomers that vary in size by one or more bases at the 3’ and/or 5’ end of the miRNA. Northern blotting for individual miRNAs showed that the proportions of isomiRs expressed by a single miRNA gene often differ between cell and tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase assays, indicating that they are functional. Bioinformatics analysis predicts substantial differences in targeting between miRNAs with minor 5’ differences and in support of this we report that a 5’ isomiR-9-1 gained the ability to inhibit the expression of DNMT3B and NCAM2 but lost the ability to inhibit CDH1 in vitro. This result was confirmed by the use of isomiR-specific sponges. Our analysis of the miRGator database indicates that a small percentage of human miRNA genes express isomiRs as the dominant transcript in certain cell types and analysis of miRBase shows that 5’ isomiRs have replaced canonical miRNAs many times during evolution. This strongly indicates that isomiRs are of functional importance and have contributed to the evolution of miRNA genes
2014-08-06 | GSE58734 | GEO