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Mus musculus

ABSTRACT: Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

PROVIDER: PRJNA146301 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR316184.fastq.gz	Fastqsanger.gz
	SRR316185.fastq.gz	Fastqsanger.gz
	SRR316186.fastq.gz	Fastqsanger.gz
	SRR316187.fastq.gz	Fastqsanger.gz
	SRR316188.fastq.gz	Fastqsanger.gz

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Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we generated a conditional knockout for this gene, specifically in cardiomyocytes. We profiled gene expression levels using RNA-seq in both normal and knockout adult mouse hearts to identify genes and pathways regulated by Tbx20. The article describing the Tbx20 knockout mouse is under review, a reference will be added when published. Analysis of triplicate mRNA samples of adult mouse, comparing normal and knockout

2012-02-10 | E-GEOD-30943 | biostudies-arrayexpress

Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

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Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding sites in the genome of 8 weeks whole heart mouse tissue. Analysis of 1 Tbx20-GFP ChIP sample of adult mouse whole heart against whole heart input

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Project description:Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

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Project description:Tbx20 is a transcription factor known to play important roles in embryonic and adult mouse heart function. Our goal in this work was to better understand the function of this gene in embryonic (E11.5) mouse cardiomyocytes that form the developing chambers, expanding our knowledge of its role in heart development. To elucidate the role of Tbx20 in mouse cardiomyocytes, we generated conditional Tbx20 knockout and compared 4 samples with 4 samples of wild-type cardiomyocytes. We found evidence of regulation of cell cycle genes by Tbx20, which are involved in proliferation. In addition, Tbx20 seems to bind and regulate an enhancer of CoupTFII in the atrium, a gene involved in atrial development.

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Project description:Genome-wide occupancy profile of Tbx3 in adult mouse whole heart

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RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function

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Project description:Purpose: The goals of this study is to determine the differential expression of transcriptome in cardiomyocyte-specific Tbx20 overexpressing adult heart (DTG) compared to controls (STG).

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The Tbx20-TLE Interaction is Essential for the Maintenance of the Second Heart Field

Project description:T-box transcription factor 20 (Tbx20) plays a multifaceted role in cardiac morphogenesis and controls a broad gene regulatory network. However, the mechanism by which Tbx20 activates and represses target genes in a tissue-specific and temporal manner remains unclear. Studies show that Tbx20 directly interacts with the Transducin-like Enhancer of Split (TLE) family of proteins to mediate transcriptional repression. However, a function for the Tbx20-TLE transcriptional repression complex during heart development has yet to be established. We created a mouse model with a two-amino acid substitution in the Tbx20 EH1 domain, thereby disrupting the Tbx20-TLE interaction. Disruption of this interaction impaired critical morphogenic events, including cardiac looping and chamber formation. Transcriptional profiling of Tbx20EH1Mut hearts and analysis of putative direct targets revealed misexpression of the retinoic acid pathway and cardiac progenitor genes. Further, we show that altered cardiac progenitor development and function contribute to the severe cardiac defects in our model. Our studies indicate that TLE-mediated repression is a primary mechanism by which Tbx20 controls gene expression.

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