Project description:Laminins are large heterotrimeric cross-shaped extracellular matrix (ECM) glycoproteins with terminal globular domains and a coiled-coil region. We have produced for the first time a recombinant laminin coiled-coil domain and studied the transcriptional profile of cells incubated in coiled-coil-coated wells.
Project description:Laminins are large heterotrimeric cross-shaped extracellular matrix (ECM) glycoproteins with terminal globular domains and a coiled-coil region. We have produced for the first time a recombinant laminin coiled-coil domain and studied the transcriptional profile of cells incubated in coiled-coil-coated wells. Cells were incubated in 24w plates, onto coiled-coil-coated wells or control BSA-coated wells (one each), 24h
Project description:We investigated the changes in gene expression profile between subconfluent (80%) and overconfluent HT-1080 fibrosarcoma cell line. We have employed whole genome microarray expression profiling as a discovery platform to identify genes expression profiles in HT-1080 cells. Total RNAs from subconfluent (HT-1080_80%) and overconfluent (HT-1080_overconfluent) HT-1080 cells were harvested and subjected to the microarray analysis.
Project description:Analysis of replication timing by RepliSeq of HT-1080 Human Connective Tissue Fibrosarcoma Cells. HT-1080 subclone +4 cell line as described in “Human gene targeting favors insertions over deletions.” Russell, D.W., and Hirata, R.K. (2008). Hum Gene Ther 19, 907-914.
Project description:Our data of mass spectrometry-based lipidomics showed that fentomycin induced the oxidation of membrane lipids in HT-1080 cells compared with the established ferroptosis inducers. To deepen this discovery, we performed quantitative mass spectrometry-based proteomics using the sublethal dose of fentomycin. The human fibrosarcoma HT-1080 cells were used. N= 5 biologically independent replicates.
Project description:A proteomic comparison between normal HT-1080 cells and HT-1080 cells that have been stably transfected to overexpress protein arginie and glutamate rich 1 (ARGLU1).
Project description:Analysis of replication timing by RepliSeq of HT-1080 Human Connective Tissue Fibrosarcoma Cells. HT-1080 subclone +4 cell line as described in “Human gene targeting favors insertions over deletions.” Russell, D.W., and Hirata, R.K. (2008). Hum Gene Ther 19, 907-914. RepliSeq of HT-1080 cell line was used to determine the impact of DNA replication on targeted adeno-associated virus sites by mapping replication forks, which revealed a consistent preference for recombination at target loci transcribed towards an incoming fork.
Project description:We investigated the changes in gene expression profile between subconfluent (80%) and overconfluent HT-1080 fibrosarcoma cell line.
