Project description:Transcriptional profiling of human umbilical vein endothelial cells following stimulation with tumour necrosis factor alpha and transforming growth factor beta singly or combined for 8 hr All stimulations were for 8 hr - TNF-α vs no cytokine; TGF-β1 vs no cytokine; TNF-α & TGF-β1 vs TNF-α alone; TNF-α & TGF-β1 vs TGF-β1 alone
Project description:TNF-alpha has a number of pro-atherogenic effects in macrovascular endothelial cells, including induction of leukocyte adhesion molecules and chemokines. We investigated the role of acyl-CoA synthetase 3 (ACSL3) in the response of cultured human macrovascular endothelial cells to TNF-alpha. TNF-alpha induced ACSL3 both in human umbilical vein endothelial cells (HUVECs) and in human coronary artery endothelial cells (HCAECs). RNA sequencing demonstrated that knockdown of ACSL3 had no marked effects on the TNF-alpha transcriptome in HCAECs. Instead, ACSL3 was required for TNF-alpha-induced lipid droplet formation from fatty acids.
Project description:Transcription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs
Project description:Transcription profiling of human umbilical vein endothelial cells overexpressing constitutively active FOXO1 to identify FOXO1 target genes
Project description:Human Umbilical Vein Endothelial Cells (HUVECs) were isolated from umbilical chords by collagenase digestion and cultured to passage 3. HUVECs were pre-activated for 4 hours with TNF-alpha (10ng/ml), followed by rHDL (0.1 and 1mg/ml) treatment for a further 8 hours. RNA isolated and labeled using the Illumina total prep RNA amplification kit and analysed using the Illumina sentrix beadchip microarray HT-12 (n=3 per treatment)
Project description:Transcription profiling of human primary osteoblasts to study the effect of direct or indirect co-culture with human umbilical vein endothelial cells
Project description:Transcriptional profiling of human umbilical vein endothelial cells following stimulation with tumour necrosis factor alpha and transforming growth factor beta singly or combined for 8 hr
Project description:Intra- and extracellular metabolomics dataset of human dermal blood endothelial cells (HDBECs), human umbilical vein endothelial cells (HUVECs), human dermal lymphatic endothelial cells (HDLECs) and intestinal lymphatic endothelial cells (iLECs) in proliferation and quiescence.
