Project description:Profiling of gene expression in Vastus Lateralis from female patients before and after GBP surgery and from lean Control Skeletal muscle samples were obtained from 5 subjects immediately before and 6 months after Gastric Bypass surgery as well as from 6 lean control subjects
Project description:Background: Primary hyperparathyroidism (pHPT) is a common endocrine disorder leading to hypercalcemia and skeletal muscle dysfunction. Muscle weakness is associated with increased risk of morbidity and mortality but is overlooked in surgical guidelines for parathyroidectomy. While parathyroidectomy is the only curative treatment, its effects on skeletal muscle strength and molecular remodelling remain underexplored. Methods: We conducted a prospective observational cohort study involving 21 postmenopausal women with pHPT, of whom 15 completed the full protocol. Clinical assessments, magnetic resonance imaging (MRI), and skeletal muscle biopsies were performed before and three months post-parathyroidectomy. Muscle strength was evaluated using the timed stand test (TST) and isokinetic dynamometry, while RNA sequencing characterized transcriptomic changes in muscle biopsies. Findings: Parathyroidectomy normalized calcium and PTH levels, accompanied by significant improvements in muscle strength and composition. MRI revealed an increase in muscle volume and a reduction in fat fraction, without changes in physical activity levels. Transcriptomic analysis identified 981 differentially expressed genes post-surgery, enriched in pathways related to extracellular matrix remodelling, angiogenesis, and mitochondrial metabolism. Notably, transcriptional changes mirrored exercise-induced adaptations.
Project description:MBD-Seq in Vastus Lateralis from male Subjects before and after GBP surgery Skeletal muscle samples were obtained from subjects immediately before and 6 months after Gastric Bypass surgery
Project description:miRNA profiles were investigated in skeletal muscle in severely obese individuals with or without diabetes before and after Roux-en-Y gastric bypass surgery.
Project description:Cancer cachexia is a multifactorial condition characterized by skeletal muscle loss that impairs longevity and quality of life of the vast majority of cancer patients. However, the ability to develop therapeutic strategies to counter cachexia is impeded by the limited understanding of the underlying mechanisms of cachexia in human cancer patients. The purpose of this study was therefore to characterize the proteomic signature of skeletal muscle obtained from cachectic pancreatic ductal adenocarcinoma (PDAC) patients, who exhibit one of the highest rates of cachexia. Muscle biopsies (rectus abdominis) were obtained from PDAC patients (n=8; 70±10yr; BMI: 26.8±5.9kg･m-2) undergoing tumor resection surgery as well as age and sex-matched non-cancer controls (n=6; 66±9yr; BMI: 30.8±5.2kg･m-2). PDAC patients were cachectic (6 month body weight loss > 5%; mean: 15.7±7.9%) and did not undergo neoadjuvant therapy.
