Project description:Ozone pollution decreases plant growth and yield worldwide. Some of the effects are genetically-mediated and are reported to involve G-protein signaling pathways. Effects of ozone on gene expression were examined in wild-type and G-protein null mutants to determine affected genes and to determine differential responses that may help define affected pathways. We used microarrays to examine changes in gene expression in response to ozone exposure and identified distinct classes of up- and down-regulated genes in wild-type and G-protein null mutant genotypes.
Project description:Ozone pollution decreases plant growth and yield worldwide. Some of the effects are genetically-mediated and are reported to involve G-protein signaling pathways. Effects of ozone on gene expression were examined in wild-type and G-protein null mutants to determine affected genes and to determine differential responses that may help define affected pathways. We used microarrays to examine changes in gene expression in response to ozone exposure and identified distinct classes of up- and down-regulated genes in wild-type and G-protein null mutant genotypes. Columbia wild-type and G-protein null mutant gpa1-4/agb1-2 plants were exposed to 5 or 125 ppb ozone in controlled environment chambers for two days. Leaves were sampled after 3 h and 2 d of exposure to examine early and late gene expression changes.
Project description:Transcriptional profiling of the vegetative part of Arabidopsis comparing wild type with the shr scl23 scr triple mutant. The latter is produced by crossing the strong null alleles of shr (shr-2), scl23 (scl23-1) and scr (scr-5). The goal was to determine the effects of the GRAS transcription factors SHR, SCL23 and SCR on growth and development of the Arabidopsis shoot system by global transcriptome analysis.
Project description:The Arabidopsis PSS1 gene, which encodes a glycine-rich protein, plays a pivotal role in nonhost resistance to the soybean oomycete Phytophthora sojae and the fungal pathogen Fusarium virguliforme. To elucidate the molecular basis of PSS1-mediated immunity, we employed miniTurbo-based proximity labeling in stable transgenic Arabidopsis lines expressing miniTurbo fused to either wild-type PSS1 or its nonfunctional mutant variant, PSS1G119D. Seedlings were challenged with or without P. sojae, and biotinylated proteins were isolated and analyzed by mass spectrometry. The resulting PSS1 interactomes revealed several candidate proteins predominantly localized in the plastid and cytosol. Notably, proteins such as LIN2, ATPMEPCRA, and TGG1 were specifically enriched in wild-type PSS1 lines under P. sojae infection, suggesting their potential involvement in PSS1-mediated nonhost resistance. Our findings offer novel insights into immune protein networks and provide a foundational resource for dissecting nonhost immunity mechanisms in plants