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Homo sapiens

ABSTRACT: Genome-wide maps of RNA Polymerase II and p65 localization in HUVECs stimulated with TNF alpha

PROVIDER: PRJNA151313 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR390742.fastq.gz	Fastqsanger.gz
	SRR390743.fastq.gz	Fastqsanger.gz
	SRR390744.fastq.gz	Fastqsanger.gz
	SRR390745.fastq.gz	Fastqsanger.gz

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Genome-wide maps of RNA Polymerase II and p65 localization in HUVECs stimulated with TNF alpha

Project description:TNF alpha is one of the inflammatory mediator and induce genes mainly by transcriptional factor, p65, in endothelial cells. This time, we performed a time course study to detect the change of localization of p65 and Pol II. To identify p65 and Pol II binding sites, we used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without TNF alpha for 30 mins. Cells were starved before stimulation longer than 16 hours. HUVECs were used within the first 6 passages. For crosslinking, 10 mM of EGS in 50% glacial acetic acid was used for 45 min, followed by 20 min of 1% paraformaldehyde treatmet was used.

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