Project description:The study outcome was to evaluate the effect of the time on normal colon mucosa samples and possibly select specific genes whose expression is time-related, that could be used as detectors of tissue degradation.
Project description:The study outcome was to evaluate the effect of the time on tumor samples and possibly select specific genes whose expression is time-related, that could be used as detectors of tissue degradation.
Project description:The study outcome was to evaluate the effect of the time on normal colon mucosa samples and possibly select specific genes whose expression is time-related, that could be used as detectors of tissue degradation. RNA extracted from specimens of 15 patients at four time points after surgery was analyzed for gene expression on high-density oligonucleotide microarrays. A mixed-effects model implemented by considering the factor time (T0, T1, T2, T3) as fixed and the factor patient as random was used to identify probes with different expression means across the four different time points. The p-values of the model were adjusted with the Bonferroni method (p<0. 05).
Project description:The regenerative capacity of the intestine declines during aging. When infected with Citrobacter rodentium, a mouse enteropathogen, young C57/BL6 mice undergo a robust regenerative response leading to survival. Old mice, however, continue to lose body weight and do not recover after infection. In this experiment, we performed a time course of the citrobacter infection in young and old mice to understand age-related differences in the intestinal response. These samples are snippets of mid-colon tissue dissected at 0, 3, 7 and 10 days post infection of young and old mice. We have 4 mice per condition.