Project description:Comparative Genomic Hybridization. Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols. Keywords: other
Project description:Comparative Genomic Hybridization. Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols.
Project description:The Bacillus cereus group consists of eight very closely related species and comprises both harmless and human pathogenic species. Yet, methods to rapidly and accurately distinguish these species are currently lacking as we demonstrate that classical matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) biotyping did not achieve reliable identification of each type strain. We assigned type strain-specific diagnostic peptides to the B. cereus group based on comparisons of their proteome profiles. The number of diagnostic peptides varies remarkably in type strain-dependent manner. The state of the art of the reference database is crucial in the process of validating candidate diagnostic peptides and may lead to a noteworthy reduction of verified diagnostic peptides as putative diagnostic peptides might be found in other species as well.
Project description:This project provides tandem mass spectrometry data of extracellular non-covalently bound cell surface proteins from samples of Bacillus cereus AH187 strain. The wild-type strain was grown on liquid MOD medium supplemented with glucose and harvested at five growth stages: early exponential, late exponential, stationary phase, tardive stationary 1 and tardive stationary 2.
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus subtilis strain 67 versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which potentially confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27.
Project description:This project provides tandem mass spectrometry datasets of Bacillus cereus ATCC 14579 wild-type strain without its pBClin15 plasmid. The strain was grown under fermentative anaerobic condition and harvested at three growth stages.
Project description:This project provides tandem mass spectrometry data of Bacillus cereus AH187 strain. The strain was grown on liquid MOD medium supplemented with glucose and harvested at three growth stages.