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Thermoanaerobacter sp. X514

ABSTRACT: Thermophilic organism isolated from a subsurface sample taken from Piceance Basin, Colorado

PROVIDER: PRJNA16394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR253126.fastq.gz	Fastqsanger.gz
	SRR4240130.fastq.gz	Fastqsanger.gz
	SRR4240140.fastq.gz	Fastqsanger.gz
	SRR4240150.fastq.gz	Fastqsanger.gz

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Similar Datasets

Thermoanaerobacter sp. X513

Project description:Thermophilic organism isolated from a subsurface sample taken from Piceance Basin, Colorado

| PRJNA32613 | ENA

Thermoanaerobacter sp. X514 in various carbon sources

Project description:The sugar metabolic mechanism on glucose, xylose, fructose and cellobiose as the sole or dual carbon source by Thermoanaerobacter sp. X514 was characterized by whole genome cDNA micorarrays. In this study, X514 cell grew in difeined medium supplement with different carbohydrates. Each sample was prepared harvested at early-, mid- and late-log phases respectively. Samples were used to acquire expression profiles of total 2322 unique genes, leading to metabolic mechanism construction.

2011-02-01 | E-GEOD-24458 | biostudies-arrayexpress

Thermoanaerobacter sp. X514 in various carbon sources

2011-02-01 | GSE24458 | GEO

Thermoanaerobacter sp. X514

Project description:the transcript profiles of Thermoanaerobacter sp. X514 (wild type) and its ethanol tolerant mutants (Xm and Xe) with or without ethanol

| PRJNA147131 | ENA

Thermoanaerobacter sp. X514

Project description:Thermoanaerobacter sp. X514 Raw sequence reads

| PRJNA810323 | ENA

Thermoanaerobacter sp. X514

Project description:Comparison of Thermoanaerobacter sp. X514 grown on different xylose concentrations

| PRJNA145511 | ENA

Thermoanaerobacter sp. X514

Project description:Thermoanaerobacter sp. X514 in various carbon sources

| PRJNA132701 | ENA

A Versatile Aldehyde: Ferredoxin Oxidoreductase from the Organic Acid Reducing <i>Thermoanaerobacter</i> sp. Strain X514.

Project description:Aldehyde:ferredoxin oxidoreductases (AORs) have been isolated and biochemically-characterized from a handful of anaerobic or facultative aerobic archaea and bacteria. They catalyze the ferredoxin (Fd)-dependent oxidation of aldehydes to acids. Recently, the involvement of AOR in the reduction of organic acids to alcohols with electrons derived from sugar or synthesis gas was demonstrated, with alcohol dehydrogenases (ADHs) carrying out the reduction of the aldehyde to the alcohol (AOR-ADH pathway). Here, we describe the biochemical characterization of an AOR of the thermophilic fermentative bacterium Thermoanaerobacter sp. strain X514 (AORX514). The putative aor gene (Teth514_1380) including a 6x-His-tag was introduced into the genome of the genetically-accessible, related species Thermoanaerobacter kivui. The protein was purified to apparent homogeneity, and indeed revealed AOR activity, as measured by acetaldehyde-dependent ferredoxin reduction. AORX514 was active over a wide temperature (10 to 95 °C) and pH (5.5 to 11.5) range, utilized a wide variety of aldehydes (short and branched-chained, aliphatic, aromatic) and resembles archaeal sensu stricto AORs, as the protein is active in a homodimeric form. The successful, recombinant production of AORX514 in a related, well-characterized and likewise strict anaerobe paves the road towards structure-function analyses of this enzyme and possibly similar oxygen-sensitive or W/Mo-dependent proteins in the future.

| S-EPMC10816221 | biostudies-literature

Characterization of the central metabolic pathways in Thermoanaerobacter sp. strain X514 via isotopomer-assisted metabolite analysis.

Project description:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose or pyruvate as both carbon and energy sources. X514 grew on minimal medium and thus contains complete biosynthesis pathways for all macromolecule building blocks. Based on genome annotation and isotopic analysis of amino acids, three observations can be obtained about the central metabolic pathways in X514. First, the oxidative pentose phosphate pathway in X514 is not functional, and the tricarboxylic acid cycle is incomplete under fermentative growth conditions. Second, X514 contains (Re)-type citrate synthase activity, although no gene homologous to the recently characterized (Re)-type citrate synthase of Clostridium kluyveri was found. Third, the isoleucine in X514 is derived from acetyl coenzyme A and pyruvate via the citramalate pathway rather than being synthesized from threonine via threonine ammonia-lyase. The functionality of the citramalate synthase gene (cimA [Teth514_1204]) has been confirmed by enzymatic activity assays, while the presence of intracellular citramalate has been detected by mass spectrometry. This study demonstrates the merits of combining (13)C-assisted metabolite analysis, enzyme assays, and metabolite detection not only to examine genome sequence annotations but also to discover novel enzyme activities.

| S-EPMC2725511 | biostudies-literature

Comparison of Thermoanaerobacter sp. X514 grown on different xylose concentrations

Project description:Thermoanaerobacter sp. X514 was grown on 1, 10, 50 and 200 mM xylose in order to identify pathways and transporters responsible for xylose transport and conversion to ethanol.

2011-11-02 | GSE31521 | GEO

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