Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs).

ABSTRACT: Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs).

PROVIDER: PRJNA171244 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

ATAC-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions

Project description:To describe chromatin accessibility changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, ATAC sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Omni-ATAC protocol was used, and each condition includes six biological replicates.�

2025-08-06 | E-MTAB-13692 | biostudies-arrayexpress

RNA-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions

Project description:To describe gene expression changes associated with differentiation of mouse presomitic mesoderm (PSM) cells into somites, RNA sequencing (paired-end) was performed using non-cultured, wild-type PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions. Smart-seq2 protocol was used, and each condition includes six biological replicates.

2025-08-06 | E-MTAB-13693 | biostudies-arrayexpress

Transcriptomic landscape of developing Presomitic mesoderm (PSM)

Project description:Stem cell-derived tissues have wide potential for modelling developmental and pathological processes as well as cell-based therapy. However, it has proven difficult to generate several key cell types in vitro, including skeletal muscle. In vertebrates, skeletal muscles derive during embryogenesis from the presomitic mesoderm (PSM). Using PSM development as a guide to establish conditions for the differentiation of monolayer cultures of embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We generated a high resolution transcriptome expression landscape along the PSM of the mouse embryo, by microdissecting consecutive fragment of the PSM along the antero-posterior axis of the embryo. We took advantage of the observation that during development of embryo, the antero-posterior spatial position of the tissue is directly correlated to its differentiation (time) stage, thus generating an expression time-course of presomitic mesoderm development.

2015-01-01 | GSE39613 | GEO

Transcriptomic landscape of developing Presomitic Mesoderm (PSM) from Tailbud to somite in E9.5 mouse embryo and in in vitro differentiated Paraxial mesoderm derived from mouse embryonic stem cells (mESCs).

2015-01-01 | GSE39615 | GEO

microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

Project description:This analysis aims at finding the microRNAs present in three chick embryo undifferentiated tissues (pools of tissues): Undetermined Presomitic Mesoderm (PSM-U), Determined Presomitic Mesoderm (PSM-D) and Distal limb bud (Limb).

2021-10-25 | E-MTAB-8176 | biostudies-arrayexpress

RNA-seq of PSM tailbud tissues isolated from wild-type and cytoPFKFB3 mouse embryos cultured in different glucose concentration

Project description:To determine glycolytic flux-responsive genes in mouse presomitic mesoderm (PSM) cells, RNA sequencing (single-end) was performed using tailbud tissues isolated from wild-type and cytoPFKFB3 PSM explants cultured in different glucose conditions. Smart-seq2 protocol was used, and each condition includes three biological replicates. This study is complementary to ENA Project PRJEB55095.

2025-08-06 | E-MTAB-13694 | biostudies-arrayexpress

Subcellular proteomics of murine presomitic mesoderm

Project description:We studied the effects of different stimuli on on the subcellular proteome of primary murine presomitic mesoderm tissue (PSM) in two separate experiments. In the first experiment, tissue was treated ex vivo with either fructose-6-phosphate, fructose-1,6-bisphosphate, or control treatment before subcellular fractionation using a subcellular protein fractionation kit (Thermo Fisher Scientific 78840). In the second experiment, PSMs from transgenic and control mice were extracted and subcellularly fractionated as above. Samples were multiplexed using TMT and analyzed on LC-MS/MS.

2022-12-07 | PXD029988 | Pride

Gene expression analysis of mouse E9.5 presomitic mesoderm wt vs TCre/+;Taf10flox/flox

Project description:Presomitic mesoderm (PSM) were microdissected from E9.5 mouse embryos (WT and TCre/+;Taf10flox/flox). 3 PSM (17-19 somites stage) were pooled per microarray, in triplicates, per genotypes

2016-10-31 | GSE82186 | GEO

microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

Project description:microRNA-Seq of Gallus gallus embryo tissues: Undetermined Presomitic Mesoderm (PSM), Determined PSM, and Limb bud

| PRJEB33661 | ENA

Bmp signaling maintains a mesoderm progenitor cell state in the mouse tailbud

Project description:Caudal somites are generated from a pool of progenitor cells located in the tailbud region. These progenitor cells form the presomitic mesoderm that gradually differentiates into somites under the action of the segmentation clock. The signals responsible for tailbud mesoderm progenitor pool maintenance during axial elongation are still elusive. Here, we show that Bmp signaling is sufficient to activate the entire mesoderm progenitor gene signature in primary cultures of caudal mesoderm cells. Bmp signaling acts through the key regulatory genes Brachyury (T) and Nkx1-2 and contributes to the activation of several other regulators of the mesoderm progenitor gene network. In the absence of Bmp signaling, tailbud mesoderm progenitor cells acquire aberrant gene expression signatures of the heart, blood, muscle and skeletal embryonic lineages. Treatment of embryos with the Bmp inhibitor Noggin confirmed the requirement for Bmp signaling for normal Brachyury expression and the prevention of abnormal lineage marker activation. Together, these results identify Bmp signaling as a non-cell autonomous signal necessary for mesoderm progenitor cell homeostasis.

2017-07-25 | GSE101810 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data