Project description:This study investigates the metabolic changes in paddy soil subjected to three different rice cultivation treatments: non-flooded unmulched cultivation (UM), non-flooded biodegradable film mulching cultivation (BM), and traditional flooding cultivation (TF). Untargeted metabolomics analysis was performed on rhizosphere soil samples collected at harvest stage using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in both positive and negative ionization modes. Raw data were acquired on a SCIEX mass spectrometer (wiff format). Soil metabolites were annotated using the Human Metabolome Database (HMDB) and KEGG pathways. This study is part of a larger investigation into the effects of biodegradable film mulching on yield, grain quality, and soil metabolic characteristics of water-saving and drought-resistant rice (variety Hanyou 73).
Project description:Paddy rice with husk can be availbale for chicken dietary resource instead of yellow corn. Ingestion of paddy rice potentially affects on gastrointestinal physiology and function including digestion/absorption of nutrients and gut barrier function such as mucosal immunity, but the details of changes is unknown. To obtain insight into the physiological modifications in the small intestine of chickens fed paddy rice, we conducted a comprehensive analysis of gene expression in small intestine by DNA microarray. In the paddy rice group, a total of 120 genes were elevated >1.5-fold in the paddy rice group, whereas a total of 159 genes were diminished < 1.5-fold. Remarkably, the gene expression levels of IGHA (immunoglobulin heavy chain α), IGJ (immunoglobulin J chain), and IGLL1 (immunoglobulin light chain λ chain region), which constitute immunoglobulin A, decreased 3 to 10 times in the paddy rice group.
