Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.
Project description:Microbes play key roles in diverse biogeochemical processes including nutrient cycling. However, responses of soil microbial community at the functional gene level to long-term fertilization, especially integrated fertilization (chemical combined with organic fertilization) remain unclear. Here we used microarray-based GeoChip techniques to explore the shifts of soil microbial functional community in a nutrient-poor paddy soil with long-term (21 years).The long-term fertilization experiment site (set up in 1990) was located in Taoyuan agro-ecosystem research station (28°55’N, 111°27’E), Chinese Academy of Sciences, Hunan Province, China, with a double-cropped rice system. fertilization at various regimes.
Project description:We present the draft genome of Nitrospirae bacterium Nbg-4 as a representative of this clade and couple this to in situ protein expression under sulfate-enriched and sulfate-depleted conditions in rice paddy soil. The proteins were extracted from the soil and analysed via LC-MS/MS measurements.
Project description:This study investigates the metabolic changes in paddy soil subjected to three different rice cultivation treatments: non-flooded unmulched cultivation (UM), non-flooded biodegradable film mulching cultivation (BM), and traditional flooding cultivation (TF). Untargeted metabolomics analysis was performed on rhizosphere soil samples collected at harvest stage using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in both positive and negative ionization modes. Raw data were acquired on a SCIEX mass spectrometer (wiff format). Soil metabolites were annotated using the Human Metabolome Database (HMDB) and KEGG pathways. This study is part of a larger investigation into the effects of biodegradable film mulching on yield, grain quality, and soil metabolic characteristics of water-saving and drought-resistant rice (variety Hanyou 73).
