Caenorhabditis elegans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA183991 | ENA |
REPOSITORIES: ENA
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Project description:Previous studies have suggested that low to moderate alcohol exposure can extend Caenorhabditis elegans (C. elegans) lifespan, but early molecular mechanisms linking ethanol exposure to longevity have not been fully characterized. Here, we investigated how ethanol treatment affects transcriptional networks in L4-stage C. elegans by time-resolved RNA-seq. L4-stage C. elegans were exposed to 5% ethanol and time-resolved RNA-seq was performed after 1, 4, and 20 hours in solution cultures, followed by differential gene expression and KEGG pathway-based Gene Set Enrichment Analysis. At 1 hour, GSEA analysis showed significant enrichment of genes in the Longevity regulating pathway (worm) with elevated expression. Core-enrichment genes exhibited coordinated upregulation of redox-defense modules, including glutathione S-transferases (gst) and p38 MAPK components (pmk-2/pmk-3), consistent with upregulation of SKN-1/Nrf2-related stress-response genes. Detoxification (gpx and fmo families) and lipid-remodeling genes were enriched at 1 hour. By 4 hours, sod-3, a canonical DAF-16/FOXO target, was clearly upregulated, suggesting engagement of DAF-16-associated antioxidant responses, whereas Peroxisome and TGF-β signaling pathways were significantly downregulated. Together, these findings provide transcriptomic insights into a temporally structured longevity-associated response to ethanol exposure, in which early detoxification and antioxidant programs, together with membrane-lipid remodeling, are followed by DAF-16-associated gene induction and repression of peroxisome- and development-related signaling. These pathway-level changes highlight candidate biological processes potentially associated with ethanol-linked lifespan modulation in C. elegans.
2026-06-04 | GSE296436 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE21008: Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans: atrazine GSE21010: Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans: cadmium GSE21011: Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans: fluoranthene Refer to individual Series
2010-03-23 | E-GEOD-21012 | biostudies-arrayexpress
Project description:Expression changes in Caenorhabditis elegans xpa-1 mutant
| PRJNA170380 | ENA
Project description:To elucidate the molecular mechanism underlying lifespan reduction induced by PM2.5 exposure in Caenorhabditis elegans, we performed global gene expression profiling by RNA-sequencing technology, and compared the gene expression pattern change induced by PM2.5 exposure.
2023-02-22 | GSE214215 | GEO
Project description:Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. Here, we study the adverse effects of GO and amino-functionalized GO (GONH2) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Seq data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. Our work establishes the enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as biomedical nanomaterial.
2022-05-10 | GSE131070 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE28617: Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq) GSE37432: Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA) Refer to individual Series
2012-06-17 | E-GEOD-37433 | biostudies-arrayexpress