Project description:Test whether it is possible to conjugate a whole plasmid library into a recipient strain without loss of fidelity (as judged by aCGH analysis)
Project description:Test whether it is possible to conjugate a whole plasmid library into a recipient strain without loss of fidelity (as judged by aCGH analysis) Donor and Recipient Libraries are compared with array CGH to detect copy number differences, a simple selection experiment is also performed
Project description:Functional defects resulting from deleterious mutations can often be restored during evolution by compensatory mutations elsewhere in the genome. Importantly, this process can generate the genetic diversity seen in networks regulating the same biological function in different species. How the options for compensatory evolution depend on the molecular interactions underlying these functions is currently unclear. This dataset, comprising multiple SATAY transposon mutagenesis libraries, was used to examine how gene deletions that compensate for defects in the polarity pathway of Saccharomyces cerevisiae impact the fitness landscape on a genome-wide scale. In total, 12 SATAY libraries were created, consisting of 6 biological replicate experiments of a wild-type strain (yEK19) and 6 biological replicates of a bem1Δbem3Δnrp1Δ mutant (yEK23). For each strain, the letter following the strain name (for example, yEK19a and yEK23a) represents different colonies taken from the same transformation plate during strain construction. Following strain construction, the different replicates of yEK19 and yEK23 were transformed with plasmid pBK549 and multiple colonies were selected from the transformation plate to generate the SATAY libraries. The numbers at the end of each strain name (for example, yEK19a_6 and yEK23a_23) indicate different colonies that were picked from the transformation after the transformation with plasmid pBK549.
Project description:In this study, we analyzed the expression profiles of a virulence plasmid-cured strain and wild-type strain of shigella flexneri. The results showed that the genes of glp regulon were upregulated in mutant bacteria in stationary phase cultures.
Project description:To further explore potential molecular mechanisms and pathways by which the presence or absence of the pGKT2 plasmid may be affecting the overall fitness cost in the native Gordonia sp KTR9 strain, transcriptome studies were performed. Transcriptome experiments comparing KTR9 wild-type and mutant strains grown in rich media confirmed the loss of the pGKT2 plasmid and also indicated the loss of the 90 kb pGKT1 plasmid.
Project description:To further explore potential molecular mechanisms and pathways by which the presence or absence of the pGKT2 plasmid may be affecting the overall fitness cost in a transconjugant Rhodococcus jostii RHA1 strain, transcriptome studies were performed. Transcriptome experiments comparing RHA1 wild-type and RHA1 transconjugant strains grown in rich media confirmed the presence of the pGKT2 plasmid.