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Mus musculus

ABSTRACT: Genome-scale DNA methylation maps of mouse chromaffin cells with or without Sdhb knock-out.

PROVIDER: PRJNA189668 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR726536_1.fastq.gz	Fastqsanger.gz
	SRR726536_2.fastq.gz	Fastqsanger.gz
	SRR726537_1.fastq.gz	Fastqsanger.gz
	SRR726537_2.fastq.gz	Fastqsanger.gz
	SRR726538_1.fastq.gz	Fastqsanger.gz

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Genome-scale DNA methylation maps of mouse chromaffin cells with or without Sdhb knock-out.

Project description:We report the analysis of DNA methylation in mouse chromaffin cell lines using reduced representation bisulfite sequencing (RRBS). We compared DNA methylation profiles of cell lines with or without a knock-out of Sdhb gene, showing that Sdhb disruption results in a hypermethylator phenotype. Reduced representation bisulfite sequencing of 4 mouse chromaffin cell samples (2 Sdhb wild-type and 2 Sdhb knock-out).

2013-05-06 | E-GEOD-44350 | biostudies-arrayexpress

Genome-scale DNA methylation maps of mouse chromaffin cells with or without Sdhb knock-out.

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Project description:Pheochromocytoma (PCC) and paraganglioma (PGL) are neuroendocrine tumours arising in the adrenal medulla and paraganglia of the autonomous nervous system, respectively. Malignant PCC/PGL are mostly caused by germline mutations of SDHB, encoding a subunit of succinate dehydrogenase. Gene expression changes associated with SDHB inactivation were investigated in a two genetically defined murine cellular models. These models were generated by Cre-mediated recombination in spontaneously immortalized mouse chromaffin cells (imCCs) and adrenal fibroblasts (MAFs).

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