Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA191088 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:While dendritic cells (DCs) are known to play a major role in the process of vaccination, the mechanisms by which vaccines induce protective immunity in humans remain elusive. Herein, we used gene microarrays to characterize the transcriptional programs induced over time in human monocyte-derived DCs (moDCs) in vitro in response to influenza H1N1 Brisbane, Salmonella enterica and Staphylococcus aureus. We built a data-driven modular analytical framework focused on 204 pathogen-induced gene clusters. The expression of these modules was analyzed in response to 16 well-defined ligands, targeting TLRs, cytoplasmic PAMP receptors and cytokine receptors. This multi-dimensional framework covers the major biological functions of APC, including the IFN response, inflammation, DC maturation, T cell activation, antigen processing, cell motility and histone regulation. This framework was used to characterize the response of monocytes and moDCs to 14 commercially available vaccines. These vaccines displayed quantitatively and qualitatively distinct modular signatures in monocytes and DCs, in particular Fluzone and Pneumovax, highlighting the functional and phenotypic differences between APC subsets. This modular framework allows the application of systems immunology approaches to study early transcriptional changes in human APC subsets in response to pathogens and vaccines, which might guide the development of improved vaccines. 60 samples of IFNa DC and 60 samples of IL4 DC grown in Media and exposed to either H1N1 Brisbane virus, heat killed Staphylococcus aureus (HKSA), heat killed Salmonella enterica (HKSE), or nothing (media control) for 1h, 2h, 6h, 12h, or 24h
2014-10-22 | E-GEOD-44720 | biostudies-arrayexpress
Project description:This experiment broadens our understanding of the temporal dynamics underlying the transcriptional response to heat stress. Using C. elegans hermaphrodite N2 populations, we have created a high-resolution time series of gene expression profiles taken at different time points during prolonged heat stress (35 C) conditions. Samples ware taken after 0h, 0.5h, 1h, 2h, 3h, 4h, 6h, 8h, and 12h of heat exposure with 3-5 biological replicas per time point.
2017-05-17 | E-MTAB-5753 | biostudies-arrayexpress
Project description:Brain transcriptome at 0h, 1h, 3h, 6h, 12h, 24h, 48h after hypoxia stress
2015-04-17 | GSE57608 | GEO
Project description:This data set provides insight into the temporal dynamics of gene expression changes during heat-stress (at 35C) and during subsequent recovery (at 20C) in Caenorhabditis elegans. Age synchronized C. elegans CB4856 hermaphrodite populations were exposed to heat stress (35C) for 0h, 0.5h, 1h, 2h, 4h, and 6h. A recovery time-series was created by using age-synchronized N2 populations exposed to heat stress (35C) for 2 hours with a subsequent recovery period of 0.5h, 1h, 1.5h, 2h, 4h, and 6h at 20C. The samples were collected at the indicated time-points during heat stress or recovery. Gene expression profiles were created using microarray technology. This data set is an extension of the data provided under accession number E-MTAB-5753 (i.e. time series describing the gene expression dynamics during heat stress of N2).
2019-09-30 | E-MTAB-7007 | biostudies-arrayexpress
Project description:There are seedling samples (high CO2 exposure of 0h, 2h, 6h, 12h, 1d, 3d, 7d, 14d) with duplicates in two chambers.
2015-12-31 | GSE49960 | GEO
Project description:We report the mRNA profiles of the Arabidopsis thaliana seedlings germinated for 7days in the dark, then treated with light for 0h, 1h, 2h, 4h, 8h and 12h.
2022-10-05 | GSE189497 | GEO
Project description:The nerve growth factor NGF has been shown to cause cell fate decisions towards either differentiation or proliferation depending on the relative activity of downstream pERK, pAKT or pJNK signaling. However, how these protein signals are translated into and fed back from transcriptional activity to complete cellular differentiation over a time span of hours to days is still an open question. Using dynamic proteome and transcriptome data from NGF-stimulated PC12 cells over a time span of 24 hours we inferred a dynamic Boolean model capturing the temporal sequence of protein signaling, transcriptional response and subsequent autocrine feedback. Optimal model topology was achieved by introducing multiple time scales for fast cellular signaling and slower gene response and subsequent fitting to the experimental data. The integrated model confirmed the parallel use of MEK/ERK, AKT/PI3K and JNK/JUN for PC12 cell differentiation. Redundancy of cell signaling is demonstrated from the inhibition of the different MAPK pathways. As suggested in silico and confirmed in vitro, differentiation was substantially suppressed under JNK/JUN inhibition, yet delayed only under MEK/ERK inhibition. Most importantly, we found that sustained transcriptional feedback is necessary to induce bistability in the cell fate switch. De novo Gene expression was necessary to activate autocrine feedback that caused integrin signaling to perpetuate the MAPK activity, finally resulting in the expression of late, differentiation related genes. Thus, the cellular decision towards differentiation depends on the establishment of a transcriptome-induced positive feedback between protein signaling and gene expression thereby constituting a robust control between proliferation and differentiation. To eludicate the transcriptome response of PC12 cells time-resolved gene expression data of NGF or EGF simulated PC12 were recorded at t = [0.5h 1h, 2h, 3h, 4h, 5h, 6h, 8h, 12h, 24h, 48h] for NGF and at t=[1h, 2h, 3h, 4h, 6h, 8h, 12h, 24h]. Control time points of unstimulated PC12 cells were taken at t=[0h, 2h, 4h, 6h, 8h, 24h, 48h]. Additionally, the MEK-inhibitor UO126 was added together with NGF and the gene response was measured at t=[1h, 2h, 4h, 6h, 8h, 12h, 24h, 48h] and for UO126 alone at t=[1h, 2h, 4h, 8h, 12h, 48h]. Total RNA was isolated, labeled and hybridized to an Illumina ratRef-12 bead array (Illumina, San Diego, CA, USA) according to the manufacturerâ??s protocol.
2016-03-23 | E-GEOD-74327 | biostudies-arrayexpress
Project description:RNA-seq analysis was performed in a TAL1-FKBP12 Jurkat cell line to analyze gene expression changes after dTAG-13 treatmentat at various time points (1h, 2h, 4h, 6h, 8h, 16h, 24h, 48h and 72h).
2023-11-20 | GSE225939 | GEO
Project description:Interventions: The patients will be divided into two groups .The first groups will receive continuous epidural infusion for analgesia and the second groups will receive continuous preperitoneal infusion for analgesia. The intervention group is the preperitoneal group in which a multiholed catheter will be placed by surgeon in the preperitoneal space at the end of surgery giving ropivacaine 0.5% 5ml/h by continuous infusion for 48 hours postoperative after giving bolus dose of 15 ml of lidocaine 2%
Primary outcome(s): postoperative pain as measured by visual analogue score
[immediate after surgery,1h,2h,4h,6h,8h,12h,18h,and24h,48h,and lastly 72h];total analgesic consumption assessed by patients medical charts[after 48 hours]
Study Design: Purpose: Treatment; Allocation: Randomised controlled trial
| 2464067 | ecrin-mdr-crc
Project description:IFNa and IL4 DCs stimulated with microbial components for 6hr
| PRJNA191087 | ENA