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Myxococcus xanthus DK 1622

ABSTRACT: Myxococcus xanthus transcriptomes for flow, liquid, and solid growth

PROVIDER: PRJNA206996 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR882090.fastq.gz	Fastqsanger.gz
	SRR882091.fastq.gz	Fastqsanger.gz
	SRR882096.fastq.gz	Fastqsanger.gz
	SRR882104.fastq.gz	Fastqsanger.gz
	SRR882110.fastq.gz	Fastqsanger.gz

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Myxococcus xanthus DK 1622

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Global profiling of gene expression during glycerol-induced spore formation in Myxococcus xanthus

Project description:Myxococcus xanthus is a gram negative rod-shaped delta proteobacterium that differentiates into environmentally resistant spores in response to starvation. Little is known about the sporulation mechanism in part because sporulation occurs in a subpopulation of cells undergoing a lenghtly complex multicellular developmental program. This developmental program requires a solid surface, motility, a minimum population density and a sophisticated network of inter and intra-cellular signals which direct some cells first to aggregate into multicellular fruiting bodies and then to sporulate exclusively within these fruiting bodies. However, it has previously been demonstrated that synchronous conversion of vegetative cells into myxospores can also be triggered in nutrient-rich liquid medium by addition of glycerol to 0.5 M. Here, we took advantage of the glycerol-induced sporulation process to gain information about the core M. xanthus sprorulation mechanism. We determined changes in the global gene expression at 0.5, 1, 2, and 4 hours after glycerol induction compared to vegetative cells (wild-type DK1622). The expression of nearly 1,500 genes was found to be significantly altered at least two-fold within four hours of glycerol-induced development. Most of the known core sporulation marker genes were up-regulated, whereas most genes required for proper aggregation and fruiting body formation were not significantly regulated. Keywords: Time course of glycerol-induced (0.5 M final conc.) development with 4 time points referenced to vegetative cells

2010-05-06 | GSE17912 | GEO

Myxococcus xanthus strain:DK1622

Project description:Myxococcus xanthus DK1622 transcriptome

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DNA regions bound by Myxococcus xanthus ParB and FrzCD

Project description:Our ChipSeq analysis show that while FrzCD does not bind DNA specific regions, ParB binds parS consistent with what as been previously shown. The FrzCD chemoreceptor from the gliding bacterium Myxococcus xanthus forms cytoplasmic clusters that occupy a large central region of the cell body also occupied by the nucleoid. FrzCD directly binds to the nucleoid and the FrzCD binding to the DNA leads to the formation of chemosensory complexes. This supra-molecular organization is required for cooperative interactions between clustered receptors, in turn important for the modulation of bacterial social behaviors.

2018-02-27 | GSE102724 | GEO

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