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Mycobacterium tuberculosis H37Rv

ABSTRACT: RNAseq Mycobacterium Tuberculosis

PROVIDER: PRJNA208118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR896652.fastq.gz	Fastqsanger.gz
	SRR896653.fastq.gz	Fastqsanger.gz
	SRR896654.fastq.gz	Fastqsanger.gz
	SRR896655.fastq.gz	Fastqsanger.gz

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Mycobacterium tuberculosis H37Rv strains: Control vs. Lupulone

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Mycobacterium tuberculosis H37Rv strains: Control vs. Lupulone

Project description:Transcriptional profiling of Mycobacterium tuberculosis H37Rv strains comparing control DMSO treated strains with Lupulone treated strains. Goal was to determine the effects of Lupulone against Mycobacterium tuberculosis H37Rv strains. Two-condition experiment,control DMSO treated strains vs. Lupulone treated strains. Biological replicates: 2 control replicates, 2 Lupulone replicates.

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Expression Analysis of WR99210-treated Mycobacterium tuberculosis H37Rv

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Arginine starvation response of Mycobacterium tuberculosis H37Rv ΔargB and ΔargF

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Expression Analysis of WR99210-treated Mycobacterium tuberculosis H37Rv

Project description:Investigation of whole genome gene expression level changes in Mycobacterium tuberculosis treated with the DHFR inhibitor WR99210, compared to untreated cells. The antimycobacterial properties of WR99210 are further described in Gerum, A., Ulmer, J., Jacobus, D., Jensen, N., Sherman, D., and C. Sibley. 2002. Novel Saccharomyces cerevisiae screen identifies WR99210 analogues that inhibit Mycobacterium tuberculosis dihydrofolate reductase. Antimicrob Agents Chemother 46(11):3362-3369 [PMID:12384337] A nine chip experiment in which total RNA was collected from H37Rv after treatment with 100 uM WR99210 for 0, 4, and 24 hours. Three separate cultures/biological replicates were interrogated for both treated and untreated cultures. Labeled cDNA was hybridized to NimbleGen spotted oligo tiling arrays covering both strands of the M. tuberculosis genome at ~200 bp intervals between 60-mer probes.

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N-terminomic Analysis of Mycobacterium tuberculosis H37Rv

Project description:Mycobacterium tuberculosis (MTB) is a species of pathogenic bacteria and the causative agent of tuberculosis. The type strain H37Rv has been sequenced in 1998, while many previous studies found its predicted genes exhibit frequent errors, particularly in start codons. Here, we applied a TMPP [(N-Succinimidyloxycarbonylmethyl) tris (2,4,6-trimethoxyphenyl) phosphonium bromide] labeling combined with StageTip separating strategy to characterize the N-terminal peptides. We identified 2,598 annotated proteins, which 1,078 proteins were labeled by TMPP.

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Mycobacterium tuberculosis H37Rv treated with VC or not

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