Project description:Transcriptional profiling of Caco-2 cells co-cultured with Faecalibacterium prausnitzii DSM17677, Lactobacilus rhamnosus HN001, UV-killed F. prausnitzii, or no bacteria in an apical anaerobic environment for four hours.
Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.
Project description:Transcriptional profiling of Caco-2 cells co-cultured with Faecalibacterium prausnitzii DSM17677, Lactobacilus rhamnosus HN001, UV-killed F. prausnitzii, or no bacteria in an apical anaerobic environment for four hours. 2 colour microarray, reference design. Biological replicates: 6 per treatment group.
Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours. Two-condition experiment with dye swap, A vs B, 6 biological replicates
Project description:Extracellular vesicles derived from milk are known to play a significant role in regulating gut microbiota. However, few studies have focused on the effects of these vesicles on specific bacterial species. This study aimed to investigate how bovine colostrum-derived extracellular vesicles (BCEVs) affect the growth and viability of commensal bacteria, specifically Akkermansia muciniphila. BCEVs and A. muciniphila were co-cultured to measure growth rates using spectrophotometry, and cell viability was assessed at the endpoints. Additionally, to determine whether BCEVs enhance the survival of A. muciniphila in the presence of Caco-2 cells, an anaerobic co-culture experiment was conducted to determine the specific interaction between intestinal epithelial cells and gut microbiota using a Transwell system. The results showed that co-culture with BCEVs increased the growth rate and viability of A. muciniphila. Consistent with this, increased viability of A. muciniphila was observed when it was co-cultured with Caco-2 cells. Transcriptomic analysis revealed that BCEVs regulate nitrogen metabolism in A. muciniphila, enhancing the growth rate and viability. Thus, regulating beneficial gut bacteria, such as A. muciniphila, through BCEVs presents a novel biological approach that positively impacts human health.
Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control).
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
